葡萄籽原花青素对哇巴因高血压大鼠血管重构的保护作用  

The protective effect of grape seed proanthocyanidin extract on vascular remodeling in ouabain-inducedhypertensive rats

作　　者：刘相菊[1] 邱洁[1] 由倍安[1] 高海青[1] 

机构地区：[1]山东大学齐鲁医院老年病科,济南250012

出　　处：《中华老年医学杂志》2013年第7期782-785,共4页Chinese Journal of Geriatrics

摘　　要：目的观察葡萄籽原花青素（GSPE）对哇巴因高血压大鼠血管重构的作用。方法30只雄性SD大鼠，随机分为3组（每组10只）：（1）对照组：每日清晨给予0．9％生理盐水1m1腹腔注射，同时0．9％生理盐水1ml灌胃；（2）畦巴因组：每日清晨给予腹腔注射哇巴因2mg／kg，并给予1ml 0．9％生理盐水灌胃；（3）GSPE组：每日清晨给予腹腔注射哇巴因2mg／kg，并给予250mg·kg^-1·d^-1GSPE灌胃。实验前、实验5周后测定大鼠血压，5周后处死大鼠，留取主动脉，通过反转录聚合酶链反应（RT-PCR）、免疫印迹分析（Westenblot）进行形态学观察和分子生物学检查。结果5周末，GSPE治疗组大鼠血压较哇巴因组下降，分别为（133．6±6．0）mmHg（1mmHg=0．133kPa）与（146．5±7．9）mmHg（P〈0．01）。形态学观察结果显示，畦巴因组大鼠主动脉内膜增厚，结构紊乱，而GSPE组与对照组血管内膜结构完整。核因子KBp65（NF-κB p65）和转化生长因子（TGF）-β1的mRNA表达及其与内参蛋白的对比中，GSPE可降低哇巴因组大鼠主动脉核因子κB（NF-κB）p65和TGF-β1的表达3．14±0．64与2．77±0．58、6．09±0．95与5．80±0．67、0．93±0．21与0．73±0．20）、0．69±0．16与0．42±0．14，均P〈0．05。结论GSPE具有拮抗内源性畦巴因的作用，可以通过抑制NF-κB和（或）TGF-β1途径，延缓了血压的升高和血管重构的过程。Objective To observe the effect of grape seed proanthoeyanidin extract （GSPE） on vascular remodeling in ouabain-induced hypertensive rats. Methods A total of 30 male Sprague- Dawley rats were randomized into 3 groups： control group （received 0.9% 1 ml normal saline by intraperitoneal injection and oral garage in the morning）, ouabain treatment group （received 2 mg/kg ouabain by intraperitoneal injection and 0.9~ 1 ml normal saline by oral gavage in the morning）, and GSPE treatment group （received 2 mg/kg ouabain by intraperitoneal injection and 250 mg Kg^-1 d^-1 GSPE by oral garage in the morning）. Blood pressure was determined before and 5 weeks after the treatment. The aortas were observed 5 weeks after the treatment. The mRNA and protein levels of nuclear faetor-±B （NF-nB p65） and transforming growth factorq31（TGF-β1） in rat aorta were detected using real-time PCR and Western blot, respectively. Morphological observations were obtained by Hemotoxylin and Eosin staining and Electron microscope. Results The systolic blood pressure was significantly lower in GSPE treatment group than in ouabain treatment group [（133.6± 6.0） mm Hg vs. （146.5±7.9） mm Hg, 1 mm Hg 0. 133 kPa, P〈0.01]. Morphological observation showed that the thickening aortic intimal and structural disorder were found in the ouabain treatment group, and aortic intimal structural integrity were normal in the other two groups. The mRNA and protein levels of NF-κB p65 and TGF-β1 were significantly lower in GSPE treatment group than in the ouabain treatment group （NF-κBp65：2.77±0.58 vs. 3. 14±0.64, 0.73±0.20 vs. 0.93±0.21, both P〈 0.05; TGF-β1： 5. 80±0. 67 vs. 6.09±0.95, 0.42±0.14 vs. 0.69±0.16, both P〈0.05）. Conclusions GSPE may inhibit endogenous ouabain, and delay the process of elevated blood pressureand vascular remodeling by inhibiting NF-κB p65 and （or） TGF-β1 1 pathways.

关 键 词：原花青素 哇巴因 高血压 

分 类 号：R285.5[医药卫生—中药学]