原发免疫性血小板减少症患者脾脏单个核细胞TIM-1及TIM-3的表达及其在Th1极化中的作用  

Expression of TIM-1 and TIM-3 in spleen mononuclear cells and their role in Thl polarization in primary immune thrombocytopenia patients

作　　者：张小梅[1] 单宁宁[1] 扈煜[2] 王欣[1] 

机构地区：[1]山东大学附属省立医院血液科,济南250021 [2]山东大学齐鲁医院化疗科

出　　处：《中华血液学杂志》2013年第7期614-617,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金（81100334）;山东省自然科学基金（ZR2010HQ002）

摘　　要：目的探讨T细胞免疫球蛋白黏蛋白分子（TIM）-1、TIM-3及Th2细胞特异性转录因子GATA结合蛋白3（GATA3）、Th1特异性转录因子T—bet在原发免疫性血小板减少症（ITP）患者脾脏单个核细胞中的表达及其临床意义。方法以17例ITP和10例外伤性脾破裂患者脾标本为研究对象。常规制备脾单个核细胞，应用SYBRw Green实时荧光定量PCR技术检测TIM—1、TIM-3、GATA3、T—bet mRNA的表达。结果ITP患者脾脏单个核细胞中TIM-3mRNA表达为正常对照组的（29＋16）％，差异有统计学意义（P〈0．05），TIM-1 mRNA表达是正常对照的（3．20±2．18）倍，差异无统计学意义（P〉0．05），TIM-1／TIM-3比值明显失衡。T—bet mRNA表达较对照组明显上升，而Th2特异性转录因子GATA3mRNA水平为对照组的14％，差异有统计学意义（P〈0．05），T—bet／GATA-3比值明显增高。结论TIM-1及TIM-3表达失衡在ITP的免疫紊乱和发生发展中起着一定的作用。Objective To explore the expression and clinical significance of T cell immunoglobulin mucin （TIM）- 1, TIM-3 and T cell-specific transcription factors T-bet and GATA-3 in spleen mononuclear cells in patients with primary immune thrombocytopenia （ITP）. Methods The spleen samples were obtained from 17 active ITP patients and 10 controls with spleen traumatic rupture. By using real-time quantitative polymerase chain reaction, the mRNA expressions of TIM-3, TIM1, T-bet and GATA-3 were studied in all subjects. Results TIM-3 mRNA levels of active ITP patients were significantly decreased to （29 ± 16）% of that of control, TIM- 1 mRNA levels of active ITP patients increased to （3.20±2.18） folds of that of control, but the difference was not significant. The ratio of TIM-1/ TIM-3 was elevated in active ITP patients. T-bet mRNA levels were up-regulated in ITP patients by （2.82± 1.57） folds （P〈0.05） and the expression of GATA3 was decreased by 14% folds （P〈0.05） compared to controls. The ratio of T-bet/GATA3 were significantly elevated in ITP patients. Conclusion The imbalance between TIM-3 and TIM-1 expression might play an important role in pathogenesis of ITP.

关 键 词：血小板减少 脾切除术 TIM-1 TIM-3 TH1细胞 

分 类 号：R558.2[医药卫生—血液循环系统疾病]