自动血细胞分析仪体液模式对脑脊液白细胞计数及分类检测的能力评估  

Assessment of the capability of the fluid model of automatic blood cell analyzer in white blood cell count and classification

作　　者：沈军[1] 陈因[1] 王国镇[1] 马亮[1] 韩呈武[1] 

机构地区：[1]中日友好医院检验科,北京100029

出　　处：《中华血液学杂志》2013年第7期629-631,共3页Chinese Journal of Hematology

基　　金：国家科技部支撑计划（2012BAH24F05）

摘　　要：目的评价XT-4000i自动血细胞分析仪在脑脊液（CSF）白细胞计数及分类应用中的价值。方法采用XT-4000i血细胞分析仪体液分析模式对200份脑脊液标本进行计数和分类分析，并与人工显微镜计数法进行结果比对。白细胞分类手工法采用瑞氏染色，仪器法采用荧光染色和流式细胞术原理进行分类。结果两种方法对脑脊液标本白细胞计数、红细胞计数、单个核及多个核细胞的绝对计数检测差异无统计学意义（P〉0．05），并且两种方法具有良好的相关性（白细胞计数r=0．987、红细胞计数r=0．999、单个核细胞绝对计数r=0．981，多个核细胞绝对计数r=0．983）。仪器检测中存在高荧光染色的标本瑞氏染色后镜检可见肿瘤细胞，与临床诊断一致。结论应用XT-4000i自动血细胞分析仪的体液模式进行脑脊液细胞计数、分类快速准确，同时还能为检测肿瘤细胞提供初筛信息。自动血细胞分析仪体液模式检测脑脊液白细胞计数及分类在临床应用中具有推广价值。Objective To evaluate the application of XT-4000i automatic blood cell analyzer in white cell count and classification of cerebrospinal fluid （CSF）. Methods The fluid model of XT-4000i automatic blood cell analyzer was directly used to detect the white cell count and classification in 200 samples of CSF, and compared with the results obtained by manually microscopic counting method. White blood cell classification was performed by manual method under microscope with Wright＇ s staining, and instrumental method with fluorescence staining and flow cytometry. Results There is no statistical difference between instrumental and manual method in detecting the absolute counting of WBC, RBC, mononuclear cell and multinucleate cells （P〉0.05） , and there is a good correlation between the two methods （r=0.987,0.999,0.981 and 0.983 for WBC, RBC, mononuclear cell and multinucleate cell counts）. Tumor cells in the samples with high fluorescent staining by instrumental method can be identified with Wright＇ s staining by microscope method, which were consistent with the clinical diagnosis. Conclusions The fluid model of XT-4000i automatic blood cell analyzer was rapid and accurate in CSF white cell count and classification, and it also can provide preliminary information for tumor cells screening. The fluid model of XT-4000i automatic blood cell analyzer in white cell count and classification of CSF has the value of popularization in clinical application.

关 键 词：脑脊液 细胞计数及分类 血细胞分析仪 手工计数 

分 类 号：R446.1[医药卫生—诊断学]