结核胸液中结核抗原刺激后CD4^+T细胞克隆性分析  

Clonality analysis of CD4^+T cells from patients with tuberculous pleurisy induced by M. tuberculosis antigens

作　　者：李丽[1] 杨滨燕[1] 劳穗华[2] 陈少华[3] 李扬秋[3] 吴长有[1] 

机构地区：[1]中山大学医学院免疫学研究所热带传染病教育部重点实验室,广州510080 [2]广州市胸科医院重症监护室,510095 [3]暨南大学医学院血液病研究所,广州510632

出　　处：《免疫学杂志》2013年第8期645-649,共5页Immunological Journal

基　　金：国家自然科学基金资助项目(31270942);广州市珠江科技新星专项(2011J2200078);广东省自然科学基金资助项目(S2011040004322)

摘　　要：目的检测结核抗原刺激后结核性胸膜炎病人胸液细胞中CD4+T细胞受体的表达情况,了解CD4+T细胞在结核局部细胞免疫应答中的作用。方法分离结核性胸膜炎病人胸液细胞(PFCs),对比BCG和结核特异性抗原(ESAT-6/CFP10混合单肽)刺激前后CD4+T细胞受体表达特征,并对刺激后部分PCR产物序列进行分析。结果 3例结核性胸膜炎患者胸液细胞未经任何刺激的条件下分别表达17~21个TCR Vβ亚家族,各存在1~2个Vβ亚家族呈现寡克隆增殖趋势。经BCG或ESAT-6/CFP10混合单肽刺激后,患者均出现4~5个寡克隆Vβ亚家族。不同患者选择性刺激的Vβ亚家族不同。结论 BCG和ESAT-6/CFP10混合单肽可分别诱导结核性胸膜炎患者不同TCR Vβ亚家族细胞寡克隆增殖,此优势表达的克隆性T细胞可能与不同结核抗原介导的特异性细胞免疫反应有关。To evaluate T cell receptor expression of CD4＋T cells in pleural fluid cells （PFCs） of patients with tuberculous pleurisy （TBP） following stimulation of Mycobacterium tuberculosis （MTB）-specific antigen, PFCs were isolated from patients with TBP, and assessed for characterization of T cell receptors after stimulation with BCG or ESAT-6/CFP-10 peptides. The sequence of some PCR products was further analyzed. We found that without any stimulation, 17-21 TCR Vβ subfamilies were detected in three patients with TBP, and 1-2 TCR Vβ subfamilies revealed to be oligoclonality. Following stimulation with BCG or ESAT-6/CFP-10 peptides, 4-5 TCR Vβ subfamilies turned to be oligoclonality. Moreover, substantial difference of TCR Vβ subfamilies was observed in various patients. In conclusion, BCG and ESAT-6/CFP-10 peptides can induce different TCR Vβ subfamilies to become oligoclonality, and the preferential expression of these TCR Vβ subfamilies may be related with the specific cellular immune responses induced by different MTB antigens.

关 键 词：CD4+T细胞 TCRVΒ基因 克隆性 

分 类 号：R392[医药卫生—免疫学]