滁菊总黄酮调控类风湿性关节炎模型大鼠滑膜组织Wnt通路SFRP4表达  

Chuju total flavonoids control the SFRP4 expression in Wnt pathway in rheumatoid arthritis model rats

作　　者：缪成贵[1,2,3] 刘健[2] 张永和[2] 高敏[2] 陈庆榆[3] 何华奇[3] 

机构地区：[1]安徽科技学院家禽疫病监测安徽省重点实验室,安徽蚌埠233100 [2]安徽科技学院食品药品学院,安徽蚌埠233100 [3]安徽科技学院生命科学学院,安徽蚌埠233100

出　　处：《中南大学学报（医学版）》2013年第7期715-721,共7页Journal of Central South University ：Medical Science

基　　金：安徽科技学院研究项目(ZRC2013378);安徽省教育厅项目(KJ2013B082)~~

摘　　要：目的:研究中药滁菊中主要活性成分总黄酮对类风湿性关节炎(rheum atoid arthritis,RA)模型大鼠滑膜组织Wnt通路分泌型卷曲蛋白4(secreted frizzled-related protein4,SFRP4)基因及SFRP4下游基因β-catenin和C-myc表达的调控作用。方法:采用大鼠关节炎评分法和足爪肿胀评分法评价滁菊总黄酮(Chuju total flavonoids,CJTF)对RA模型大鼠的治疗作用;RT-PCR和Western印迹分别检测CJTF灌胃治疗对模型大鼠滑膜组织Wnt通路中SFRP4,β-catenin和C-myc基因mRNA和蛋白表达的调控作用。结果:经CJTF灌胃治疗后,模型大鼠关节炎评分、足爪肿胀评分均明显下降。模型大鼠滑膜组织中SFRP4基因mRNA和蛋白表达明显上调,而其下游基因β-catenin和C-myc基因mRNA和蛋白表达明显下调。结论:CJTF对RA模型大鼠有明显的治疗作用,其机制与以SFRP4为靶点,抑制RA模型大鼠滑膜组织Wnt通路的激活有关。Objective： To determine the effect of Chuju total flavonoids （CJTF） on the secreted frizzled- related protein 4 （SFRP4） expression in Wnt pathway in rheumatoid arthritis （RA） model rats. Methods： The role of CJTF in the treatment of RA model rats was evaluated by rat arthritis score and paw edema score. The expression regulation of the SFRP4, β-catenin and C-myc in Wnt pathway in RA model rats was detected by RT-PCR and Western blot after CJTF gavage treatment. Results： After CJTF treatment, the rat arthritis score and paw edema score in RA model rats weresignificantly decreased when the RA model rats were treated with CJTF, the SFRP4 expression was significantly up-regulated, while theβ-catenin and C-myc gene expression were significantly down- regulated in RA model rat synovial tissues. Conclusion： CJTF has significant therapeutic effect and inhibitory effect on Wnt pathway activation by targeting SFRP4 in RA model rat synovium.

关 键 词：滁菊总黄酮 类风湿性关节炎 滑膜组织 分泌型卷曲蛋白4 WNT Β-CATENIN 

分 类 号：R285.5[医药卫生—中药学]