瘢痕性气道狭窄的血清蛋白质组学研究  

Serum proteomic analysis of cicatricial airway stenosis

作　　者：王利换[1] 张杰[1] 王娟[1] 王婷[1] 张莹莹[1] 徐敏[1] 

机构地区：[1]首都医科大学附属北京天坛医院呼吸内科,100050

出　　处：《中华结核和呼吸杂志》2013年第7期506-509,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的对比瘢痕性气道狭窄患者与健康对照组的血清蛋白质指纹图谱，寻找与气道瘢痕增生有关的蛋白。方法应用表面增强激光解析电离飞行时间质谱（SELDI—TOF—MS）检测17例瘢痕性气道狭窄患者和17名健康人血清蛋白质图谱，用BiomarkerWizard软件分析得到两组间差异蛋白。结果瘢痕性气道狭窄患者血清蛋白图谱与对照组相比，存在49个差异表达蛋白（P〈0．05），其中25个高表达，24个低表达，经蛋白质数据库搜索，初步确立特定相对分子质量所对应的蛋白。结论瘢痕性气道狭窄患者血清和对照组血清中存在明显差异蛋白，这些差异蛋白可能与气道瘢痕的发生发展密切相关。Objective To establish serum protein fingerprint profile in patients with cicatricial airway stenosis and compared with healthy control. Methods Serum samples of 17 cicatricial airway stenosis patients and 17 healthy persons were analyzed by SELDI-TOF-MS to select the differently expressed proteins through Biomarker Wizard software. Results Compared with healthy control ,49 protein biomarkers were identified. Among them ,25 proteins were up-regulated ,24 proteins were down-regulated. These proteins were confirmed by searching database. Conclusions There are obvious differentially expressed proteins in patients with cicatricial airway stenosis and controls, which may related with the development of airway scar.

关 键 词：气道狭窄 表面增强激光解析电离飞行时间质谱 蛋白质组学 

分 类 号：R562[医药卫生—呼吸系统]