三七粉对实验性慢性硬膜下血肿病理特征与VEGF及其受体表达的影响  

Effect of Panax Notoginseng Powder on Pathological Features and Expressions of VEGF and Its Receptors of Chronic Subdural Hematoma Rabbits: an Experimental Study

作　　者：刘建辉[1] 邢海亭 王垣芳[2] 栾海云[2] 杨茗[2] 

机构地区：[1]烟台市烟台山医院神经外科,山东烟台264001 [2]滨州医学院药学院,山东烟台264003

出　　处：《中国中西医结合杂志》2013年第7期938-943,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30901979);山东省高校科技计划项目(No.J09LF58);烟台市科学技术发展计划项目(No.2011233)

摘　　要：目的探讨三七粉对慢性硬膜下血肿(chronic subdural hematoma,CSDH)模型家兔病理特征的影响及相关机制。方法硬膜下少量多次注血法制备家兔CSDH模型,造模成功后随机分成模型组、三七低剂量(0.125g/kg)组、三七高剂量(0.250g/kg)组,每组6只;模型组给予生理盐水,三七组给予三七粉,连续灌胃6天。HE染色观察血肿外膜病理组织学特征;化学比色法测定外膜SOD活力和MDA含量;免疫组织化学法观察外膜CD31、CD34及VEGF表达;ELISA法检测外周血和硬膜下血肿液VEGF表达;Westernblot检测血肿外膜VEGFR-1和VEGFR-2蛋白表达。结果与模型组比较,三七低、高剂量组血肿外膜炎症反应较轻而纤维组织增生相对成熟;高剂量组外膜SOD活性增高(P<0.05),外膜CD31、CD34表达降低(P<0.01),硬膜下残液VEGF表达降低(P<0.05);三七低、高剂量组外膜VEGF、VEGFR-2表达降低(P<0.05,P<0.01)。与三七低剂量组比较,高剂量组CD31、CD34表达量降低(P<0.01),高剂量组VEGFR-2表达减少(P<0.05)。结论三七能加快CSDH血肿局部纤维性修复和病程演变;减轻外膜炎症和氧化损伤、降低VEGF表达并通过影响VEGFR-2受体途径减少病理性血管形成可能是其重要机制。Objective To observe the effect of Panax notoginseng （PN n chronic subdural hematoma （CSDH） rabbits and its mechanisms. Methods ） on pathological features A stable pathological animal model similar to CSDH in humans could be established using subdural injections of small number of blood through a subdural pre-catheter in rabbits. After successful modeling, 18 rabbits were randomly di- vided into the model group, the low dose PN group （0. 125 g/kg）, and the high dose PN group （0.250 g/kg）, 6 in each group. Normal saline was given to rabbits in the model group, while PN power was given to those in the PN groups by gastrogavage for 6 successive days. Pathologic features of the hematoma outer membrane were observed by HE staining. The activity of SOD and the content of MDA in the hematoma outer membrane were examined by the colorimetric method. Expressions of CD31, CD34, and VEGF in the hematoma outer membrane were observed by immunohistochemical assay. Expressions of VEGF in the peripheral blood and the subdural hematoma were detected by enzyme-linked immunosorbent assay （ELISA）. Expressions of VEGFR-1 and VEGFR-2 in the hematoma outer membrane were detected by Western blot. Results Compared with the model group, the inflammatory reaction was com- paratively lessen and the proliferation of the fibrous tissue was relatively mature in the low and high dose PN groups. The activity of SOD increased （P 〈0.05） ; expressions of CD31 and CD34 were reduced （P 〈 0.01 ） ; VEGF expression in the residual hematoma fluid decreased （P 〈0.05） in the high dose PN group. Expressions of VEGF and VEGFR-2 were all reduced in the high and low dose PN groups （P 〈0.05, P 〈 0.01）. Compared with the low dose PN group, expressions of CD31 and CD34 were reduced （P 〈0.01 ）, and the VEGFR-2 expression was also reduced （P 〈0.05） in the high dose PN group. Conclusions PN could promote the fibrous repairing of subdural hematoma in CSDH rabbits. It also lessened inflammation and oxidative inj

关 键 词：慢性硬膜下血肿 三七粉 血管内皮生长因子 血管内皮生长因子受体-1 血管内皮生长因子受体-2 

分 类 号：R651.15[医药卫生—外科学]