三七总皂苷对快速老化模型小鼠大脑β-分泌酶活性及含量的影响  

Effect of PNS on the Activity and Content of BACE1 in the Brain of SAMP8 Mice with Alzheimer′s Disease

作　　者：黄金兰[1] 陆璐[2] 黄丹[2] 吴登攀[3] 钟振国[1] 

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西中医药大学新药开发中心,南宁530001 [3]中山大学基础医学院,广州510275

出　　处：《中国中西医结合杂志》2013年第7期944-947,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30960489;No.81160512);广西自然科学基金项目(No.2010GXNSFD013050)

摘　　要：目的研究三七总皂苷(Panax notoginseng saponin,PNS)对快速老化小鼠亚系8(senescence accelerated mouse-prone 8,SAMP8)小鼠大脑β-分泌酶(Beta-site amyloid precursor protein cleavage e-zyme,BACE,主要是BACE1)活性及含量的影响。方法选择SAMP832只,随机分为对照组、PNS高剂量组(200mg/kg)、PNS低剂量组(100mg/kg)和石杉碱甲组(0.3mg/kg),每组8只,对照组给予等容积双蒸水灌胃。每天灌胃1次,连续给药2个月。分别采用直接荧光法和Westernblot法检测SAMP8大脑BACE1的活性和BACE1蛋白含量。结果 PNS高低剂量组(高剂量组:2.008±0.031;低剂量组:2.221±0.029)和石杉碱甲组(2.267±0.076)相对荧光单位(RFU/μg)值均比对照组(2.403±0.058)低(P<0.01)。PNS高低剂量组(高剂量组:0.900±0.028;低剂量组:1.000±0.032)和石杉碱甲组(0.837±0.080)BACE1蛋白表达均比对照组低(2.210±0.074,P<0.01)。结论高于100mg/kg的PNS可以降低SAMP8脑内BACE1活性,下调BACE1蛋白表达。Objective To explore the effect of Panax notoginseng saponin （PNS） on the activity and content of β-secretase in the brain of senescence accelerated mouse-prone 8 （SAMPS） mice with Alzheimer＇s disease. Methods Totally 32 SAMP8 mice were randomly divided into the normal control group, the high dose PNS group （200 mg/kg）, the low dose group （100 mg/kg）, and the huperzine A group （0.3 mg/kg）, 8 in each group. Equal volume of double distilled water was given to those in the normal control group. All medication was given by gastrogavage, once daily for two successive months. The activity of BACE1 was assayed by direct immunofluorescent method （DIF）. The content of BACE1 protein was detected by Western blot. Results The relative fluorescence units （RFU/μg） was 2.008 ± 0.031 in the high dose PNS group, 2.221±0.029 in the low dose PNS group, and 2.267 ±0.076 in the huperzine A group, all lower than that in the normal control group （2.403 ±0.058 ; all P 〈0.01 ）. The con- tent of BACE1 protein was 0.900 ±0. 028 in the high dose PNS group, 1. 000±0.032 in the low dose PNS group, and 0.837±0.080 in the huperzine A group, all lower than that in the normal control group （2.210 ±0.074, all P 〈0.01）. Conclusion PNS higher than 100 mg/kg could decrease the activity of BACE1 and down-regulate the content of BACE1 protein in the brain of SAMP8 mice.

关 键 词：三七总皂苷 快速老化SAMP8小鼠 Β-分泌酶 

分 类 号：R285.5[医药卫生—中药学]