针刺对重症急性胰腺炎早期急性肺损伤大鼠血清MIP-2蛋白及肺与大肠组织MIP-2 mRNA表达的影响  

Effect of Acupuncture on Serum MIP-2 and MIP-2 mRNA Expressions in Isolated Fei and Dachang of Severe Acute Pancreatitis Induced Acute Lung Injury Rats in the Acute Phase

作　　者：蒋莉娅[1] 黄继人 赵弘卿[3] 诸静芬[4] 戴建良 张卫东[4] 

机构地区：[1]无锡市第二人民医院中医科,江苏无锡214002 [2]无锡市中西医结合医院肝胆胰中心,江苏无锡214041 [3]无锡市第二人民医院呼吸科,江苏无锡214002 [4]无锡市中医院普外科,江苏无锡214001

出　　处：《中国中西医结合杂志》2013年第7期958-962,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2009CB522702)

摘　　要：目的观察针刺对重症急性胰腺炎(severeacute pancreatitis,SAP)早期急性肺损伤(acute lung injury,ALI)大鼠血清巨噬细胞炎症-2(macrophage inflammatory protein-2,MIP-2)蛋白及肺与大肠组织MIP-2mRNA表达的影响。方法 40只雄性Wistar大鼠随机分为假手术组、SAP组、针刺治疗组和对照组,每组10只。以3.5%牛黄胆酸钠逆行注射胰胆管的方法制作SAP模型,针刺治疗组在"肺与大肠相表里"的理论指导下,于SAP造模0.5h后行针刺肺、大肠、脾经穴位及其背腧穴;针刺对照组在造模0.5h后行针刺肺、脾经穴位及其背腧穴。观察造模6h后各组大鼠血清肿瘤坏死因子(tumor necrosis factoralfa,TNF-α)、一氧化氮(nitric oxide,NO)及MIP-2蛋白表达水平,应用RT-PCR法检测大鼠肺与大肠组织MIP-2mRNA表达水平。结果 SAP组6h后血清TNF-α、NO、MIP-2蛋白表达水平及肺与大肠组织MIP-2mRNA表达水平均明显高于假手术组(P<0.05),针刺治疗组各指标均低于SAP组及针刺对照组(P<0.05)。针刺治疗组、SAP组及针刺对照组大鼠MIP-2蛋白及mRNA间均呈正相关(P<0.05)。结论 "肺与大肠相表里"理论指导下的针刺对SAP大鼠早期急性肺损伤保护作用明显,其作用机制可能与通过抑制肺与大肠组织MIP-2mRNA的过度表达,降低血清MIP-2蛋白表达水平有关。Objective To observe effect of acupuncture on serum macrophage inflammatory protein-2 （MIP-2） and MIP-2 mRNA expressions in isolated Fei and Dachang of severe acute pancreatitis （SAP） induced acute lung injury （ALl） rats in the acute phase. Methods Forty male Wistar rats were randomly divided into four groups, i.e., the sham-operation group, the SAP group, the acupuncture treat- ment group, and the acupuncture control group, 10 in each group. The SAP model was induced by retro- grade infusion of 3.5% sodium taurocholate into the pancreatobiliary duct. Under the guidance of ＂Fei and Dachang exterior-interiorly related＂, points were acupunctured along Fei, Dachang, and Pi channels, as well as those points on the back of rats in the acupuncture treatment group 0.5 h after modeling. Be- sides, points were acupunctured along Fei and Pi channels, as well as those points on the back of rats in the acupuncture control group 0.5 h after modeling. Serum levels of tumor necrosis factor （~ （TNF-（x） and nitric oxide（NO）, and MIP-2 expressions were examined 6 h after modeling. Expressions of MIP-2 mRNA in isolated lung and large intestine tissues were detected by reverse transcription PCR. Results Com- pared with the sham-operation group, serum levels of TNF-（x and NO, and expressions of MIP-2 and MIP- 2 mRNA in isolated lung and large intestine tissues were significantly higher in the SAP group （P 〈0.05）. Each index was lower in the acupuncture treatment group than in the SAP group and the acupuncture con- trol group （P 〈0.05）. Besides, the serum level of MIP-2 and the MIP-2 mRNA expression in isolated lung and large intestine tissues were positively correlated in all groups except the sham-operation group （P 〈 0.05）. Conclusions Under the guidance of ＂Fei and Dachang exterior-interiorly related＂, acupuncture could remarkably reduce the severity of SAP induced AL related to suppressing over-expressions of MIP-2 mRNA lowering the serum MIP-2 expression level. rats in the acut

关 键 词：针刺 胰腺炎 急性肺损伤 肺 大肠 巨噬细胞炎症蛋白-2 

分 类 号：R245[医药卫生—针灸推拿学]