印迹基因H19和Igf2在子宫内膜异位症患者子宫内膜组织中的异常表达及其意义  

Expression of imprinted gene H19 and Igf2 in the endometrium of patients with endometriosis

作　　者：王俊豪[1,2] 阮钰[1,2] 李蓉[1] 

机构地区：[1]北京大学深圳医院生殖医学科,深圳518036 [2]汕头大学医学院,汕头515041

出　　处：《生殖医学杂志》2013年第7期523-526,共4页Journal of Reproductive Medicine

基　　金：高等学校博士学科点专项科研基金(20090001110104)

摘　　要：目的研究印迹基因H19和Igf2在子宫内膜异位症(内异症)患者子宫内膜的表达及临床意义。方法选择2011年1月至2012年8月至北京大学深圳医院生殖中心行试管助孕并已行宫腹腔镜检查的患者,其中证实为内异症的患者31例作为实验组,因男方因素不孕、女方因素完全正常的妇女33例作为对照组,收集分泌中期子宫内膜组织,采用实时定量聚合酶链反应方法(RT-PCR)测定两组子宫内膜组织H19和Igf2 mRNA表达水平。结果 H19和Igf2 mRNA在内异症患者子宫内膜组织中的平均表达量分别为49.909±46.970和8.747±5.451,在对照组中的平均表达量分别为19.728±23.990和0.513±0.973,H19和Igf2在内异症患者与正常女性子宫内膜组织中的表达差异均具有统计学意义(P=0.006和0.00)。结论印迹基因H19和Igf2可能与内异症性不孕有关。Objective： To investigate expression of imprinted gene H19 and Igf2 in the endometrium of patients with endometriosis. Methods： Among the patients who received hysteroscopy for in vitro firtilization in Department of Reproductive Medicine in Peking University Shenzhen Hospital from Jan. 2011 to Aug. 2012,31 patients with endometriosis were recruited as experimental group,and 33 normal women whose infertility was due to male factor were as control group. The endometrial tissue samples were collected in their mid-secretary phase. The expressions of H19 and Igf2 in the endometrium were measured by real-time quantitative polymerase chain reaction. Results： The average expression levels of H19 and Igf2 in the endometrium of patients with endometriosis were 49. 909±46. 970 and 8. 74±5. 451 respectively,while they were 19. 728±23. 990 and 0. 513 ±0. 973 respectively in the endometrium of normal women. There was significant difference between the two groups in the expression H19 and Igf2 gene（P=0. 006 and 0.00 respctively）. Conclusions： Imprinted gene H19 and Igf2 may be relevant to endometriosis.

关 键 词：印迹基因 H19 IGF2 子宫内膜异位症 

分 类 号：R4[医药卫生—临床医学]