红景天苷通过抑制凋亡相关蛋白的表达保护缺氧对培养神经干细胞的损伤  

Salidroside protects cultured rat subventricular zone neural stem cells against hypoxia injury by inhibiting Bax, Bcl-2 and caspase-3 expressions

作　　者：祁存芳[1,2] 张军峰[1,3] 陈新林[1] 张建水[1] 杨蓬勃[1] 焦倩[1] 张蓬勃[1,4] 吕海侠[1] 刘勇[1] 

机构地区：[1]安交通大学医学院//教育部环境和基因相关疾病重点实验室//神经生物研究所,陕西西安710061 [2]青海大学医学院基础医学部解剖学教研室,青海西宁810001 [3]西安医学院人体解剖学教研室,陕西西安710021 [4]西安交通大学医学院第二附属医院麻醉科,陕西西安710004

出　　处：《南方医科大学学报》2013年第7期962-966,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30960107,81070998,31070943)~~

摘　　要：目的观察红景天苷对缺氧损伤成年大鼠侧脑室室管膜下区(SVZ)组织分离培养神经干细胞凋亡比例和Bax、Bcl-2、caspase-3蛋白表达的影响。方法原代培养成年大鼠SVZ神经干细胞,不同浓度红景天苷预处理24 h后,干细胞厌氧工作站缺氧损伤48 h。四甲基偶氮唑盐(MTT)法检测各组细胞活力;显微镜下观察各组细胞形态;TUNEL染色和流式细胞仪检测细胞凋亡情况;Western blot检测细胞内Bax,Bcl-2和caspase-3蛋白的表达变化。结果红景天苷预处理可显著改善缺氧损伤后的细胞生长状态和活力,降低缺氧引起的细胞凋亡率的升高(P<0.05);拮抗缺氧损伤导致的细胞Bcl-2/Bax蛋白比例的下降,降低活化的caspase-3蛋白的表达(P<0.05)。结论红景天苷通过抑制神经干细胞的凋亡,保护缺氧对神经干细胞的损伤,其机制可能与调节凋亡相关基因Bax、Bcl-2和caspases-3的表达有关。Objective To explore the effects of salidroside （sal） on the expressions of Bcl-2, Bax and caspases-3 proteins in cultured rat subventricular zone （SVZ） neural stem cells （NSCs） exposed to hypoxia injury. Methods Primarily cultured SVZ NSCs from adult SD rats were incubated with salidroside （120 and 240 μmol/L） for 24 h prior to exposure to hypoxia. The cell viability was assessed with MTT assay, and the cell apoptosis was analyzed using TUNEL staining and flow cytometry. Western blotting was performed to detect the expressions of Bcl-2, Bax and caspase-3 in the cells. Results Salidroside pretreatment of the cells for 24 h resulted in an obvious resistance to hypoxia-induced cell apoptosis and decrement of cell viability （P〈0.05）. Salidroside also antagonized the effect of hypoxia exposure in lowering Bcl-2/Bax ratio apoptosis of rat neural stem cells and decreased the expression of caspases-3 protein （P〈0.05）. Conclusion Salidroside can significantly resist hypoxia-induced. The neuroprotective effect of salidroside may be related to the modulation of expressions of apoptosis-related proteins.

关 键 词：红景天苷 缺氧 神经干细胞 BAX Bcl-2 CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]