四种消毒方式对简易呼吸囊的灭菌效果评价  

Sterilizing effect and residual bacteria of four different methods for sterilizing simple breathing vesicles

作　　者：陆爱武[1] 王媚[2] 聂志强[3] 郭远波[1] 王春晓[1] 

机构地区：[1]广东省人民医院麻醉科,广东广州510080 [2]广东省人民医院细菌室,广东广州510080 [3]广东省人民医院心外科流行病研究室,广东广州510080

出　　处：《南方医科大学学报》2013年第7期1067-1070,共4页Journal of Southern Medical University

基　　金：广东省科技计划项目(2010B080702034)

摘　　要：目的研究观察4种不同消毒方式对简易呼吸囊的消毒效果和微生物残留状况,探讨加强呼吸囊消毒方式方法管理的必要性。方法分4组消毒试验:G1组43例,500 mg/L二氧化氯(ClO2)喷雾;G2组28例,酒精喷雾;G3组47例,50 mg/L三氯异氰尿酸(TCCA)浸泡;G4组46例,50 mg/L ClO2溶液浸泡。各组于消毒后30 min取样接种培养,菌落计数后用卡方秩和进行统计学分析,并对残留菌鉴定分型。结果(1)4组菌落计数有显著性差异(P<0.001)。细菌残留率:G1组为0%,G2组53.6%,G3组27.7%,G4组21.7%,各组间比较统计学有显著性差异(P<0.001)。(2)残留菌鉴定结果显示铜绿假单胞杆菌和饱曼不动杆菌,溶血性葡萄球菌等常见耐药性致病菌。结论麻醉简易呼吸囊以500 mg/L ClO2喷雾方式消毒消果最好。中低效TCCA反复浸泡长期使用,可能导致耐药致病菌生长。Objective To study the effects of 4 different methods for disinfection of simple breathing vesicles and microbial residue. Methods The disinfection tests were divided into 4 groups： G1 group （43 cases） with 500 mg/L chlorine dioxide spray, G2 group （28 cases） with alcohol spray, G3 group （47 cases） with 50 mg/L trichloro isocyanuric acid （TCCA） immersion, and G4 group （46 cases） with 50 mg/L chlorine dioxide solution immersion. Afrer 30 min of dsinfection, each group was examined by bacterial culture and colony count. The residual bacteria were identified and typed. Results The 4 methods showed significant differences in bacterial colony count （P〈0.001）. The rate of bacterial residue was 0% in G1 group, 53.6% in G2 group, 27.7% in G3 group, and 21.7% in G4 group, showing significant differences between the 4 groups （P〈0.001）. The residual bacteria included antibiotic- resistant common opportunistic pathogen such as Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus haemolytic. Conclusions Disinfection with 500 mg/L chlorine dioxide spray is the best for simple breathing vesicles. Prolonged immersion in TCCA may lead to the growth of drug-resistant pathogens in the breathing vesicles.

关 键 词：简易呼吸囊 消毒 二氧化氯 三氯异氰尿酸 耐药致病菌 

分 类 号：R187[医药卫生—流行病学]