尼古丁对大鼠骨折愈合过程中骨痂含量变化及成熟度的影响  

Impact of nicotine on rat fracture healing callus content and process maturity

作　　者：戴冠东 刘东云[1] 黄伟忠[1] 徐勇[1] 吴爱国[1] 刘国辉[2] 

机构地区：[1]深圳市坪山新区人民医院骨科,深圳518118 [2]华中科技大学同济医学院附属协和医院骨科

出　　处：《中国医师杂志》2013年第3期300-303,共4页Journal of Chinese Physician

基　　金：深圳市科技计划项目（201103329）

摘　　要：目的探讨尼古丁对大鼠骨折愈合过程中骨痂含量变化及成熟度的影响。方法60只雄性SD大鼠，按随机数字表法分为模型组、轻度尼古丁组、重度尼古丁组，每组各20只，造模前对三组大鼠分别皮下注射0．5ml／（kg·d）生理盐水、2mg／（kg·d）尼古丁、4mg／（kg·d）尼古丁连续3周。制造大鼠桡骨3mm的横断骨折愈合模型；于术后3、7、14、21d每组随机处死5只大鼠，取左侧桡骨标本作为观察对象，使用HE染色法检测骨痂厚度及成熟度。结果造模后第3天，轻度尼古丁组、重度尼古丁组与模型组比较，骨痂厚度差异无统计学意义（P〉0．05），显微镜下骨痂成熟度无差异；造模后第7天模型组、轻度尼古丁组、重度尼古丁组骨痂厚度分别为（1．59±0．09）mm、（1．43±0．12）mm、（1．39±0．09）mm，第14天各组骨痂厚度分别为（1．98±0．12）mm、（1．78±0．08）mm、（1．68±0．09）mm，第21天各组骨痂厚度分别为（2．39±0．09）mm、（1．93±0．11）mm、（1．89±0．09）mm，各给药组与模型组比较，骨痂厚度差异有统计学意义（P〈0．05，P〈0．01）；给药组骨痂厚度及骨痂成熟度较模型组低。结论尼古丁影响骨痂增殖分化使骨痂含量减少及骨痂成熟延迟，而延缓骨折的愈合过程。Objective To explore the effect of nicotine on rats callus content and maturity in the process of fracture healing. Methods Sixty male Sprague-Dawley rats were randomly divided into model group, mild nicotine group and severe nicotine group （ n = 20/each group）. The 3 -mm bone defects fracture models were made in the junction of the lower 1/3 of the rat left radial. Five rats of each group were sacrificed randomly in the 3, 7, 14, 21 days after surgery, respectively. The left radial were collected as the observed object. The callus thickness and maturity of the specimens were detected by HE staining. Results At the 3rd days after modeling,the difference in specimens callus thickness between each treatment group and the model group was not statistically significant （ P 〉0. 05 ）, no difference in the maturity of the callus under the microscope; callus thickness in mild and. severe nicotine groups and model group was （ 1.59 ± 0. 09 ） mm, （ 1.43 ± 0. 12 ）mm, （ 1.39 ±0. 09 ） mm at the 7th day after modeling, （ 1.98 ± 0. 12 ） mm, （ 1.78 ± 0. 08 ） mm and （ 1.68 ± 0. 09 ）mm at the 14th day after modeling, and （ 2. 39 ± 0.09 ） mm, （ 1.93 ± 0. 11 ） mm, （ 1.89 ± 0. 09 ）mm at the 21 st day after modeling; The difference of callus thickness in specimens between each treatment group and the model group had statistical significance （ P 〈 0. 05, P 〈 0. 01 ） , callus thickness and maturity of the treatment group were lower than that in the model group. Conclusions Nicotine affects the proliferation and differentiation of callus, reduces callus formation, inhibits maturity transformation of bone, and delays the healing process of fracture.

关 键 词：尼古丁 药理学 骨折愈合 骨痂 

分 类 号：R6[医药卫生—外科学]