丙酮酸乙酯对缺氧缺血性脑损伤新生大鼠脑组织的保护作用  

Protective effect of ethyl pyruvate on brain tissues in neonatal rats with hypoxic-ischemic brain damage

作　　者：欧阳颖[1] 苏浩彬[1] 薛红漫[1] 吴燕云[1] 方建培[1] 

机构地区：[1]中山大学孙逸仙纪念医院儿科,广东广州510120

出　　处：《中国病理生理杂志》2013年第7期1181-1185,共5页Chinese Journal of Pathophysiology

基　　金：广东省医学科学技术研究基金(No.A2010161)

摘　　要：目的:探讨丙酮酸乙酯(ethyl pyruvate,EP)对缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)新生大鼠脑组织的作用及其可能机制。方法:165只7日龄SD大鼠随机分为3组:假手术组(n=43)、HIBD模型组(n=61)和HIBD+EP处理组(n=61),造模前30 min腹腔注射EP(50 mg/kg)1次,此后每天1次,3 d后测定脑组织匀浆超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量以及脑组织含水量,TUNEL法检测脑组织凋亡细胞数;14 d后检测缺血侧和非缺血侧脑重量以判别脑萎缩程度。结果:HIBD+EP组脑组织SOD活性为(125.78±18.35)×103 U/(g protein),较HIBD模型组[(97.84±15.50)×103 U/(g protein)]明显增强(P<0.05);MDA含量为(4.42±1.04)μmol/(g protein),较HIBD模型组[(6.02±0.89)μmol/(g protein)]明显降低(P<0.05)。此外,HIBD模型组缺血侧(左侧)的脑组织含水量高于非缺血侧(右侧)(P<0.05),EP治疗后两侧的脑组织含水量无显著差异(P>0.05),提示EP减轻了缺血侧的脑水肿。同时HIBD+EP组缺血侧脑皮质和海马每个视野的凋亡细胞数目分别为96.63±10.08和41.91±9.96,较模型组(111.54±1.64和51.73±1.77)明显减少,但仍多于假手术组(P<0.05)。HIBD+EP组左脑萎缩程度为(13.25±5.19)%,较HIBD模型组[(20.32±5.10)%]明显减轻(P<0.05)。结论:EP具有抗氧化功能,能减轻脑水肿,减少脑细胞凋亡,减轻脑萎缩,对HIBD新生大鼠脑组织具有保护作用。AIM： To study the effects of ethyl pyruvate（EP） on brain tissues in neonatal rats with hypoxic-ischemic brain damage（HIBD） and its underlying mechanisms.METHODS： A total of 165 seven-day-old Sprague-Dawley（SD） rats were randomly divided into 3 groups： sham operation group（n = 43）,HIBD group（n = 61） and HIBD ＋ EP group（n = 61）.The rats in HIBD ＋ EP group were intraperitoneally injected with EP（50 mg / kg） 30 min before operation,and once a day after surgery.Superoxide dismutase（SOD） activity and malondialdehyde（MDA） content in brain homogenate,water content of brain and apoptotic cells in cortex were detected 3 days later.Ischemic and non-ischemic brain tissues were weighed to assess the extent of brain atrophy 14 days later.RESULTS： Higher level of SOD [（125.78 ± 18.35） × 103 U /（g protein） ] and lower level of MDA [（4.42 ± 1.04） μmol /（g protein） ] in HIBD ＋ EP group than that in HIBD group [（97.84 ± 15.50） × 103 U /（g protein） and（6.02 ± 0.89） μmol /（g protein）,respectively]was observed（P 0.05）.In addition,the water content of ischemic hemisphere was significantly higher than that of non-ischemic one in HIBD group（P 0.05）,and was indistinguishable from that of non-ischemic one after EP treatment（P 0.05）,indicating the protective effect of EP against brain edema.The apoptotic cells in cortex and hippocampus in HIBD ＋ EP group [（96.63 ± 10.08） / field and（41.91 ± 9.96） / field,respectively] were obviously decreased compared with HIBD group [（111.54 ± 1.64） / field and（51.73 ± 1.77） / field,respectively],but still higher than those in sham operation group（P 0.05）.The atrophy ratio of ischemic hemisphere in HIBD ＋ EP group was（13.25 ± 5.19） %,significantly lower than that in HIBD group [（20.32 ± 5.10） %,P 0.05].CONCLUSION： Ethyl pyruvate is neuroprotective against HIBD in neonatal rats via increasing SOD level,decreasing MDA level,attenuating brain edema,decreasing apoptotic

关 键 词：缺氧缺血性脑损伤 丙酮酸乙酯 神经保护作用 

分 类 号：R722.1[医药卫生—儿科]