Chloropyrula uraliensis gen. et sp. nov. （Trebouxiophyceae, Chlorophyta）, a new green coccoid alga with a unique ultrastructure, isolated from soil in South Urals  

作　　者：Lira GAYSINA Yvonne NEMCOVA Pavel SKALOUD Tereza SEVCIKOVA Marek ELIAS 

机构地区：[1]Department of Botany, Bioecology and Landscape Design, Bashkir State Pedagogical University named after M. Akmullah, Oktyabrskoy Revolucii St., 3a, Ufa 450000, Russia [2]Faculty of Science, Department of Botany, Charles University in Prague, Benatska 2, Prague 128 01, Czech Republic [3]Faculty of Science, Department of Biology and Ecology, University of Ostrava, Chittussiho 10, Ostrava 710 00, Czech Republic

出　　处：《Journal of Systematics and Evolution》2013年第4期476-484,共9页植物分类学报（英文版）

基　　金：Acknowledgements We thank Jiri NEUSTUPA, Igor KOSTIKOV, Tatiana MIKHAILYUK, and Tatyana DARIENKO for valuable discussions, two anonymous reviewers for their valuable comments on the original version of the manuscript, and Lenka FLASKOVA for excellent technical help. This work was supported by the Grant No. P506/10/0705 from the Czech Science Foundation, the Project No. CZ.1.05/ 2.1.00/03.0M. 100 （Operational Program Research and Development for Innovations）, and the internal grant 11.01. of the Bashkir State Pedagogical University named after M. Akmullah.

摘　　要：Soil hosts diverse communities of photosynthetic eukaryotes （algae） that have not yet been fully explored. Here we describe an interesting coccoid green alga isolated from a soil sample from a forest-steppe in South Urals （Bashkortostan, Russia） that, based on a phylogenetic analysis of 18S rRNA gene sequence, appears to represent a new phylogenetic lineage related to the genus Leptosira within the class Trebouxiophyceae. This new alga is characterized by uninucleate cells with a shape ranging from spherical to ellipsoid or egg-like, occurring solitary or more often grouped in irregular masses or colonies. Remarkably, cells with a characteristic pyriform shape are encountered in cultures grown on a solid medium. The cells harbour a single pyrenoid-lacking parietal chloroplast with the margin undulated or forming finger-like projections; in mature cells the chloroplast becomes divided by deep incisions into more or less separate lobes. Transmission electron microscopy of vegetative cells revealed an unprecedented structure in the form of a cluster of microfibrils located in the cytoplasm near the plasma membrane, often appressed to the chloroplast. Reproduction takes place via autospores or biflagellated zoospores. The unique suite of characters of our isolate distinguishes it from previously described coccoid green algae and suggests that it should be classified as a new species in a new genus; we propose it be named Chloropyrula uraliensis.Soil hosts diverse communities of photosynthetic eukaryotes （algae） that have not yet been fully explored. Here we describe an interesting coccoid green alga isolated from a soil sample from a forest-steppe in South Urals （Bashkortostan, Russia） that, based on a phylogenetic analysis of 18S rRNA gene sequence, appears to represent a new phylogenetic lineage related to the genus Leptosira within the class Trebouxiophyceae. This new alga is characterized by uninucleate cells with a shape ranging from spherical to ellipsoid or egg-like, occurring solitary or more often grouped in irregular masses or colonies. Remarkably, cells with a characteristic pyriform shape are encountered in cultures grown on a solid medium. The cells harbour a single pyrenoid-lacking parietal chloroplast with the margin undulated or forming finger-like projections; in mature cells the chloroplast becomes divided by deep incisions into more or less separate lobes. Transmission electron microscopy of vegetative cells revealed an unprecedented structure in the form of a cluster of microfibrils located in the cytoplasm near the plasma membrane, often appressed to the chloroplast. Reproduction takes place via autospores or biflagellated zoospores. The unique suite of characters of our isolate distinguishes it from previously described coccoid green algae and suggests that it should be classified as a new species in a new genus; we propose it be named Chloropyrula uraliensis.

关 键 词：Chloropyrula uraliensis green algae new species TREBOUXIOPHYCEAE ULTRASTRUCTURE Ural Mountains. 

分 类 号：Q959.223.5[生物学—动物学] X833[环境科学与工程—环境工程]