烟碱降解菌的筛选、鉴定及其降解性能的初步研究  

A preliminary study on the screening,identification and degradation characteristics of nicotine degradation bacterium strain

作　　者：徐慧[1,2] 党立志[1] 杨根华[2] 王明锋[1] 夏建军[1] 李青青[3] 段焰青[1] 

机构地区：[1]红云红河烟草(集团)有限责任公司技术中心,云南昆明650202 [2]云南农业大学植物保护学院,云南昆明650201 [3]云南师范大学生命科学学院,云南昆明650092

出　　处：《云南大学学报（自然科学版）》2013年第4期580-586,共7页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金项目(NSFC31160432);云南省科技厅项目(2012BA015);云南中烟工业公司项目(2011CP02;2010Fl01);红云红河集团自立项目(HYHH2012HX05;HYHH2012HX06)

摘　　要：以烟碱为唯一碳氮源,分别从云南省5个地区(腾冲、文山、弥勒、石林、玉溪)新鲜、健康烟草叶片表面及叶内分离得到18株烟碱降解菌,进一步筛选得到2株降解活性较高的菌株.经常规的形态观察,生理生化分析和16S rDNA序列同源性分析,初步鉴定2株菌同属于亚种(Pseudomonas cedrina subsp.cedrina).在温度28℃,培养基初始pH=7.0,摇床转速为180 r/min的培养条件下,当烟碱质量浓度低于3 g/L时,18 h即可将烟碱完全降解.该2株菌的烟碱降解周期较已报道同属或其他降解菌短,降解效率较高,具有较好的工业应用前景,且此亚种首次被报道具有降解烟碱功能.Eighteen nicotine - degrading bacteria were isolated from fresh and healthy tobacco leaves collected from 5 counties （ Tengchong, Wenshan, MiLe, Shilin and Yuxi） in Yunnan Province, and two strains which showed higher nicotine - degrading abilities were identified as Pseudomonas cedrina subsp, cedrina based on their 16S rDNA sequences,morphological and biochemical characteristics. The two strains could utilize nicotine as the sole source of carbon and nitrogen,degrading nicotine （ 〈3.0 g/L） within 18 h at 28 ℃ and pH =7.0. The nicotine - degrading cycle was shorter than other previously reported bacteria with the same abilities. Therefore, the two stains have potential industrial prospects in nicotine- degrading. It is the first time of Pseudomonas cedrina subsp, cedrina being reported for degrading nicotine.

关 键 词：烟碱 生物降解 假单胞菌 云南 

分 类 号：TS414[农业科学—烟草工业]