白术挥发油提取工艺的优化及其化学成分研究  

Optimize the extraction process of volatile oil from rhizoma atractylodis macrocephalae and study its chemical composition

作　　者：黄小千[1] 牟洁 朱莹[3,4] 贺嵩敏[3,4] 冯兵[3,4] 李秀丽[1] 郑广娟[3,4,1] 

机构地区：[1]山东中医药大学 [2]山东医学高等专科学校,山东济南250002 [3]广东省中医院,广东广州510006 [4]广东省中医药科学院,广东广州510006

出　　处：《山东中医药大学学报》2013年第4期339-342,共4页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省科技厅重大项目(编号:2008GG30002027)

摘　　要：目的:确定水蒸气蒸馏法提取白术挥发油的最佳工艺并研究其化学成分。方法:采用正交试验设计对提取条件进行优化,并通过气相色谱-质谱联用仪(GC-MS)分析挥发油化学成分。结果:以得油率为指标,结合经济省时的原则评价其最佳工艺为浸泡时间1 h,蒸馏时间5 h,加水量5倍,得油率为4.23‰。GC-MS分析所得挥发油,共分离出47个成分,鉴定了其中32个成分。结论:本实验优化了水蒸气蒸馏法提取白术挥发油的提取工艺,并通过GC-MS分析了所提挥发油的化学成分,为白术挥发油的开发利用提供可靠参考。Objective：To determine optimum process of the water vapor distillation extraction of volatile oil from rhizoma atractylodis macrocephalae and study its chemical composition.Methods：Using the orthogonal experimental design,the extraction conditions were optimized,and through the GC-MS analysis volatile oil chemical composition.Results：Combining the oil rate,the economy and saving time,it indicated the optimum process as follow：to soak time 1 h,distillation time 2 h,add water 5 times.The oil rate was 4.23‰.Using GC/MS,a total of 47 chromatographic peaks were separated and 32 chromatographic peaks were identified.Conclusion：Through water vapor distillation,this experiment to optimize the extracting process of rhizoma atractylodis macrocephalae of the volatile oil.The chemical constituents of volatile oil can be analysed through GC-MS.This experiment provide reliable reference for further development and utilization of rhizoma atractylodis macrocephalae of the volatile oil.

关 键 词：白术 水蒸气蒸馏法 正交试验设计 挥发油 GC-MS 苍术酮 

分 类 号：R284.1[医药卫生—中药学]