HPLC法测定炎痢净片中芍药苷的含量及溶出度  

Content and Dissolution Determination of Paeoniflorin in Yanlijing Tablets by HPLC

作　　者：邹小艳[1] 欧彬娟[1] 周意[2] 陈祖辉[1] 马宁[1] 

机构地区：[1]长沙医学院,长沙410219 [2]江西中医学院

出　　处：《中国药师》2013年第7期987-990,共4页China Pharmacist

摘　　要：目的:建立测定炎痢净片中芍药苷的含量及溶出度的方法。方法:采用SinoChron ODS-BP色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸(14:86),流速1.0 ml·min^(-1),检测波长285 nm,柱温35℃。结果:芍药苷在0.41～41.20μg·ml^(-1)范围内线性关系良好(r=0.999 9),平均回收率为99.6%(RSD=1.80%,n=6)。结论:该方法简便、可靠,可作为炎痢净片中芍药苷的含量及溶出度测定。To establish a method for the content and dissolution determination of paeoniflorin in Yanlijing tablets. Method：A SinoChron ODS-BP column（250 mm×4.6 mm,5μm）was used.The mobile phase was acetonitrile-0.1%phosphoric acid（14：86）.The flow rate was 1.0 ml·min^（-1）.The UV detection wavelength was 285 nm,and the column temperature was 35℃. Result：The linear range of paeoniflorin was 0.41-41.20μg·ml^（-1）（r = 0.999 9）,and the average recovery was 99.6%（RSD = 1.80%,n =6）.Conclusion：The method is simple and reliable,and can be used in the content and dissolution determination of paeoniflorin in Yanlijing tablets.

关 键 词：高效液相色谱法 炎痢净片 芍药苷 含量 溶出度 

分 类 号：R927.2[医药卫生—药学]