可注射性壳聚糖/β-甘油磷酸钠凝胶负载富含血小板血浆和骨髓基质干细胞促进牙周再生的研究  

The effect of injectable chitosan/β-glycerol phosphate gel combined with platelet-rich plasma and bone marrow mesenchymal stem cells in repairing periodontal defects

作　　者：吴广升[1] 惠光艳[1] 关继东[2] 刘丽霞 陈丽洁[1] 贾文敏[1] 

机构地区：[1]济南军区青岛第一疗养院,266071 [2]青岛大学医学院 [3]青岛崂山区卫生服务中心

出　　处：《实用口腔医学杂志》2013年第4期468-471,共4页Journal of Practical Stomatology

基　　金：青岛市科技支撑计划(编号:10-3-3-7-1-nsh)

摘　　要：目的:观察可注射性壳聚糖/β-甘油磷酸钠(chitosan/β-glycerol phosphate disodium salt,C/GP)凝胶负载富含血小板血浆(platelet-rich plasma,PRP)和骨髓基质干细胞(bone marrow mesenchymal stem cells,BMSCs)修复牙周组织缺损的效果。方法:选取3只健康雄性杂种犬,制备前磨牙及第一磨牙区共30颗牙的Ⅱ度根分叉区缺损模型。随机分为A组(空白对照组)、B组(C/GP)、C组(C/GP+PRP组)、D组(C/GP+BMSCs)和E组(C/GP+PRP+BMSCs组)。术中先严密缝合各组牙周缺损处组织瓣,将凝胶注射入B至E组相应牙周缺损模型处,A组不作处理。术后8周取材行X射线及组织学观察。结果:C、D、E组与A、B组相比均出现明显的牙周组织再生(P<0.05),E组与C、D组相比牙周组织再生最显著(P<0.05)。A组和B组仅有少量的牙周组织再生,二者相比没有统计学差异(P>0.05)。结论:C/GP凝胶同时负载PRP和BMSCs可有效促进牙周组织再生。Objective：To observe the effect of the injectable chitosan/β-glycerol phosphate disodium salt（C/GP） hydrogel loading platelet-rich plasma（PRP） and bone marrow mesenchymal stem cells（BMSCs） in repairing periodontal defects.Methods： ClassⅡfurcation defect models were induced surgically in 30 teeth of the second and third premolars and mandibular first molars in 3 healthy male dogs and the teeth were divided into 5 groups at random.In group A,the defects were not treated.In group B,C,D and E the defects were filled with C/GP hydrogel,C/GP/PRP,C/GP/BMSCs and C/GP/PRP/BMSCs respectively.The dogs were sacrificed at 8weeks after surgery and the samples were prepared for X-ray and histologic examination.Results： More periodontal tissue regeneration was observed in group C,D and E than in group A and B（P〈0.05）,significantly greater periodontal regeneration was found in group E than in group C and D（P〈0.05）.No significant difference was found between group A and B（P〈0.05）.Conclusion： The C/GP hydrogel loading with PRP and BMSCs can effectively promote the periodontal tissue regeneration.

关 键 词：可注射性 壳聚糖 富含血小板血浆 骨髓基质干细胞 牙周组织再生 

分 类 号：R318.08[医药卫生—生物医学工程]