平邑甜茶延长根和吸收根抗凋亡基因的表达差异及其对2,4-D的响应  

Differential Expression of Anti-apoptotic Genes in Extensive and Absorbing Roots of Malus hupehensis and Their Response to 2,4-D

作　　者：樊树雷[1] 杨洪强[1] 冉昆[1] 沈伟[1] 张玮玮[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018

出　　处：《园艺学报》2013年第7期1225-1232,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31171923);国家转基因生物新品种培育重大专项(2008ZX08009-3);高校博士学科点专项科研基金项目(20123702130001;20103702110003)

摘　　要：以3年生平邑甜茶[Malus hupehensis(Pamp)Rehd.var.pinyiensis Jiang]盆栽树为材料,通过 2,4-D水溶液灌根处理,探讨了新根(延长根和吸收根)细胞死亡及其抗凋亡基因的表达特征。结果表明,在60mg·L-12,4-D处理后的70d内,新根细胞死亡量和类 caspase3/7(半胱天冬酶)活性先上升后下降,ATP含量和根系活力的变化与之相反。平邑甜茶抗凋亡基因 MhBAG、MhBI-1 与 MhHSP70 在新根中均受 2,4-D 诱导,随着2,4-D处理时间的延长,MhBAG基因的表达水平先下降后逐渐升高,MhBI-1与MhHSP70则先升高后下降。在2,4-D处理下,吸收根细胞死亡量与根系活力的变化幅度明显高于延长根,吸收根MhBI-1与MhHSP70表达量的早期升高幅度大于延长根,表明吸收根细胞对2,4-D 的敏感性高于延长根;但在基因表达高峰时,延长根抗凋亡基因表达量的升高倍数明显高于吸收根,暗示延长根比吸收根有更强的抗凋亡能力。The three-year old potted trees ofMalus hupehensis （Pamp） Rehd. var. pinyiensis Jiang were irrigated with water solution of 2,4-D to investigate the characteristics of cell death and the expression of anti-apoptotic genes of new roots （extensive roots and absorbing roots） . The result showed that the new roots＇ cell death quantity and caspase-like 3/7 activity increased first and then fell in the 70 days after the treatment of 60 mg. L-1 2,4-D, however, the ATP content and the root activity changed in the contrary trend. After the treatment, the expression ofanti-apoptotic genes MhHSP70, MhBI-1, MhBAG were induced and the expression levels of MhBAG first decreased then increased, however MhHSP70 and MhBI-1 increased first, and then decreased later. At the same time, the change range of cell death quantity and caspase-like 3/7 activity and the rising range of MhHSP70 and MhBI-1 expression levels in absorbingroots were greater than that in extensive roots in the early stage of the 2,4-D treatment, which showed that absorbing roots had higher sensitivity to 2,4-D than extensive roots. When at peak value, the expression levels rising times of anti-apoptotic genes in extensive roots were significantly higher than that in absorbing roots, which indicated that the extensive roots had stronger anti-apoptotic ability than absorbing roots.

关 键 词：平邑甜茶 2 4-D 延长根 吸收根 抗凋亡基因 细胞死亡 

分 类 号：S661[农业科学—果树学]