同种大鼠骨髓间充质干细胞诱导调节性B淋巴细胞的免疫负调节作用  

An experimental study on the role of MSCs in inducing regulatory B cells and the underlying mechanisms

作　　者：王月[1] 牛坚[1] 

机构地区：[1]徐州医学院附属医院普通外科,江苏省徐州市221002

出　　处：《中华器官移植杂志》2013年第7期428-431,共4页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金项目资助，江苏省333人才项目

摘　　要：目的研究骨髓间充质干细胞（MSC）诱导调节性B淋巴细胞（Breg细胞）的免疫负调节作用。方法取DA大鼠MSC和Lewis大鼠B淋巴细胞共培养后，酶联免疫吸附试验（EUSA）检测MSC和B淋巴细胞混合培养组上清液中白细胞介素10（IL-10）的水平和分泌因子的变化；采用流式细胞技术分析B淋巴细胞增殖、凋亡、表型的变化和对抗原特异性T淋巴细胞增殖的影响，采用激光共聚焦显微镜观察Breg细胞对免疫复合物吞噬能力的变化。结果MS（2成功诱导产生高分泌IL-10的Breg细胞产生，表型为CDl9＋CDld＋CD5＋，MSC显著抑制B淋巴细胞的增殖，Breg细胞能抑制抗原特异性T淋巴细胞的增殖，并增加对免疫复合物的吞噬能力。结论MS（2诱导的Breg细胞具有明显的免疫负调节作用。Objective To observe the immunoregulatory roles of MSCs in inducing proliferation of B regulatory cells （Bregs）. Method DA Rat MSCs were co-cultured with Wistar rat B ceils. The levels of IL-10 and secrete proinflammatory cytokines in the supematants were determined by using ELISA. The proliferation and apoptosis of B cells after interaction with MSCs were analyzed by using FACS. In order to investigate whether Bregs are involved in the phagocytosis of immune complex （IC）, B ceils and Bregs were incubated with FITC-OVA IC. The fluorescence intensity was detected by using immunofluorescence microscopy. Results MSCs induced B cells to differentiate into Bregs with high CD19, CDld and CD5 expression and high IL-10 level. The proliferation of B cells was significantly suppressed after interaction with MSCs. Bregs had enhanced phagocytic capacity as compared with that of B cells. Bregs significantly inhibited maDC-induced CD4＋ T cell proliferation compared to B cells, without depressing IL-2 or IFN-7 secretion. Conclusion MSCs induce freshly isolated B cells to differentiate into Bregs characterized by the ability to preferentially produce IL-10.

关 键 词：大鼠 间质干细胞 B淋巴细胞 免疫耐受 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]