红景天对AOPPs诱导小鼠足细胞转分化的影响  

Effect of Salidroside on Mouse Podocytes Epithelial-mesenchymal Transition Induced by Advanced Oxidation Protein Products

作　　者：章俊[1] 华洁[1] 马亚琼[1] 刘蓉芝[1] 邱敏姿[1] 杨蕾[1] 汤珣[1] 

机构地区：[1]南方医科大学珠江医院肾内科,广州510280

出　　处：《中国中西医结合肾病杂志》2013年第4期305-308,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：广东省科技计划项目(No.2007B031503001);广州市科技计划项目(No.11C22120703);广东省自然科学基金资助项目(No.S2011010004053)

摘　　要：目的:探讨红景天(salidroside)对晚期氧化蛋白产物(AOPPs)诱导小鼠足细胞转分化的影响。方法:以小鼠永生性足细胞系为研究对象。给予200μg/ml的AOPP刺激小鼠足细胞24h,同时加入红景天(浓度分别为0.1μmol、1μmol、10μmol、100μmol、200μmol)进行干预,采用Westernblot检测内质网应激标志性蛋白Grp78、CHOP以及平滑肌激动蛋白(α-SMA)、nephrin、podocin的表达水平。结果:200μg/mlAOPP条件下小鼠足细胞表达高水平的Grp78、CHOP及α-SMA,表达低水平的nephrin和podocin;予不同浓度红景天作用后,足细胞中Grp78、CHOP及α-SMA的表达水平降低,neph-rin和podocin的表达水平升高。结果表明红景天可部分逆转AOPPs的作用,且存在一定的剂量依赖性。结论:红景天对AOPPs诱导的足细胞Grp78、CHOP过度表达有很好的抑制作用,这可能是它减轻肾脏纤维化的机制之一。Objective：To investigate the effect of Salidroside on Mouse Podocytes Epithelial-mesenchymal transition（EMT） induced by Advanced Oxidation Protein Products（AOPP）.Methods：Using mice glomerular podocyte cell line as an in vitro system,podocytes were stimulated by AOPPs（200 μg/ml） and Salidroside（0.1 μmol,1 μmol,10 μmol,100 μmol,200 μmol）for 24 hours.Then the cells were collected and expression of alpha-smooth muscle actin（α-SMA）,Grp78（glucose-regulated protein78）,CHOP,nephrin and podocin were detected by Western blot.Results：Under AOPPs（200 μg/ml） condition,there were high expressions of Grp78,CHOP and α-SMA,and low expression of nephrin and podocin in mice podocyte cell.After the treatment with Salidroside（0.1 μmol,1 μmol,10 μmol,100 μmol,200 μmol）,the expressions of Grp78,CHOP and α-SMA were decreased and the expression of nephrin and podocin were increased in mice podocyte cell.The results showed that Salidroside could partly reverse the effect of AOPPs in a dose-dependent manner.Conclusion：Salidroside can attenuate renal fibrosis,and the protective mechanism may be partly through by regulating the over expressions of genes Grp78 and CHOP.

关 键 词：AOPPs 小鼠足细胞 转分化 红景天 

分 类 号：R285.5[医药卫生—中药学]