糖肾宝含药血清对高糖培养的大鼠肾小球系膜细胞增殖的影响及机制研究  

Effect of Serum Containing Tangshenbao on the Proliferation of Rat Glomerular Mesangial Cells Induced by High Glucose and Its Mechanism

作　　者：向少伟[1] 黄露[1] 黄仁发[1] 程新[1] 吴潇芸[1] 黄海燕[1] 

机构地区：[1]广西中医药大学附属瑞康医院,南宁530011

出　　处：《中国中西医结合肾病杂志》2013年第4期309-312,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：广西壮族自治区卫生厅基金资助项目(No.Z2009197)

摘　　要：目的:探讨中药复方糖肾宝(主要由黄芪、大火草、葛根、莪术、三七组成)对高糖培养的大鼠系膜细胞(MCs)增殖的影响及可能作用机制。方法:体外培养大鼠MCs,随机分为正常组、高糖对照组、糖肾宝低、中、高剂量组、厄贝沙坦组。除正常组外,其余各组分别予以30mmol/L葡萄糖+正常大鼠血清、糖肾宝低、中、高剂量血清、厄贝沙坦血清进行干预48h。MTT法检测细胞增殖,RT-PCR法检测单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)mRNA表达,ELISA检测MCP-1蛋白,western印迹检测TGF-β1蛋白表达。结果:高糖作用下的各组MCs增殖、MCP-1和TGF-β1表达均显著高于正常组(P<0.05,P<0.01);药物干预的各组MCs增殖、MCP-1和TGF-β1表达均显著低于高糖对照组(P<0.05,P<0.01);糖肾宝高剂量组MCs增殖、MCP-1和TGF-β1表达与厄贝沙坦组差异无统计学意义(P>0.05)。结论:中药复方糖肾宝能抑制高糖培养的MCs细胞增殖,其机制可能与抑制炎症反应有关。Objective：To investigate the effect of Tangshenbao Granule on the proliferation of rat glomerular mesangial cells（MCs） induced by high glucose and its mechanism.Methods：The rat MCs were randomly divided into normal group（normal rat serum）,high glucose control group（30 mmol/L glucose and normal rat serum）,Tangshenbao low（30 mmol/L glucose and serum containing low dose Tangshenbao）,middle（30 mmol /L glucose and serum containing middle dose Tangshenbao） and high-dose（30 mmol /L glucose and serum containing high dose Tangshenbao） group,irbesartan group（30 mmol /L glucose and serum containing irbesartan）.Cell proliferation was detected by MTT.TGF-β1 and MCP-1 mRNA were observed by real-time PCR.Expression of MCP-1 protein was examined by Enzyme-Linked Immunosorbnent Assay.Expression of TGF-β1 protein was examined by Western blot.Results：Compared with normal group,in all groups induced by high glucose the proliferation of MCs,MCP-1and TGF-β1 were significantly higher（P〈0.05,P〈0.01）;Compared with high glucose control group,in all intervention groups the proliferation of MCs,MCP-1and TGF-β1 were significantly lower（P〈0.05,P〈0.01）;Compared with irbesartan group,in Tangshenbao high-dose group the proliferation of MCs,MCP-1 and TGF-β1 expression was not significantly different（P〈0.05）.Conclusion：Tangshenbao can inhibit the proliferation of MCs induced by glucose,and its mechanism may be related to inhibiting inflammatory reaction.

关 键 词：糖尿病肾病 糖肾宝 细胞培养 单核细胞趋化蛋白-1 转化生长因子-Β1 

分 类 号：R285.5[医药卫生—中药学]