利用凝集素芯片检测多囊肾病特异iPS细胞表面糖谱的研究  

A Method of Profiling ADPKD iPS Cells on Lectin Microarray

作　　者：周树敏[1] 黄佳卉[1] 胡斌[1] 牛鑫[1] 郭尚春[1] 汪年松[2] 汪泱[1] 

机构地区：[1]上海交通大学附属第六人民医院四肢显微外科研究所,上海200233 [2]上海交通大学附属第六人民医院肾内科,上海200233

出　　处：《中国中西医结合肾病杂志》2013年第6期491-493,I0002,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国家自然科学基金资助项目(No.81170640)

摘　　要：目的:建立一套凝集素芯片检测活体iPS细胞表面糖谱的方法。方法:以ADPKD-iPS为对象,运用不同活细胞染料,尝试不同的染色时间,找到最佳的iPS细胞与凝集素芯片结合的实验方案。结果:利用SYBR green,染色30min可以获得最佳的信号强度和信噪比。结论:已成功建立了一套利用凝集素芯片对活体iPS细胞进行表面糖谱检测的方法。Objective：To develop a method on screening the membrane glycomics of iPS using lectin microarray. Methods：ADPKD-iPS was employed as a model to be tested by stained with diverse fluorescent dyes and staining time, in order to find the best one which is suitable to lectin microarray incubation. Results：As a result, ADPKD-iPS stained with SYBR green I for 30 min at 37℃ is the best method to label iPS cells. Conclusion：We have established a novel method of profiling live iPS cells on lectin microarray.

关 键 词：多囊肾 IPS 凝集素芯片 表面糖谱 

分 类 号：R692.1[医药卫生—泌尿科学]