糖尿病大鼠膀胱重构中M_3受体改变的实验研究  

Bladder Remodeling and Changes of M_3 Muscarinic Receptors in the Type 2 Diabetes Mellitus Rats

作　　者：高宏飞[1] 王东文[1] 

机构地区：[1]山西医科大学第一医院泌尿外科,太原030001

出　　处：《中国中西医结合肾病杂志》2013年第6期494-497,共4页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国家自然科学基金资助项目(No.30972987);山西省回国留学人员科研基金资助项目(No.2007-47);山西省青年科技研究基金资助项目(No.2012021034-1)

摘　　要：目的:研究病程24周的2型糖尿病大鼠膀胱重构时,逼尿肌收缩功能的改变和M3受体含量及其基因转录水平的改变情况,并探讨二者之间的相关性。方法:2d龄雌性Wistar大鼠随机分成实验组和正常对照组,应用链脲佐菌素腹腔注射并结合高糖高脂饮食进行2型糖尿病大鼠动物模型制备。于糖尿病病程24周时进行下列实验:应用离体膀胱灌注方法观察逼尿肌收缩功能的变化;应用RT-PCR和Western blotting方法观察逼尿肌M3受体mRNA和蛋白表达的变化。结果:2型糖尿病组大鼠逼尿肌收缩功能低于正常对照组,为(16.52±2.97)cmH2O/100mgVS(25.66±3.56)cmH2O/100mg;2型糖尿病组大鼠逼尿肌M3受体mRNA和蛋白的表达均高于正常对照组,分别为(65.27±4.61)%VS(37.53±4.02)%和(45.19±2.37)%VS(23.67±2.85)%。结论:本研究证实了2型糖尿病大鼠在病程24周时膀胱逼尿肌的收缩力降低,但M3受体的生物合成却上调,这种不平行现象可能是病变进展的表现。Objective：To study the changes of detrusor contraction and M3 muscarinic receptors in the type 2 diabetes mellitus rats. Methods：The type 2 diabetic rats model was established by intraperitoneal injection of streptozotocin （STZ） and high caloric diet. The experiments were carried through at the 24-week of the course of type 2 diabetes mellitus. The contractile functions of the whole bladder was measured by bladder filling experiments in vitro. The expression of M3 muscarinic receptor mRNA and protein of the whole bladder tissue were detected by RT-PCR and western blotting methods. Results：The whole bladder filling experiments showed that the bladder contractile function of the 24-week diabetic rats was decreased when compared with the control rats by about （16.52±2.97） VS （25.66±3.56）cm H2O/100 mg. The RT-PCR and western blotting showed that the expression of M3 muscarinic receptor mRNA and protein of the 24-week diabetic rats groups were increased than the control groups by about （65.27±4.61）% VS （37.53±4.02）% and （45.19±2.37）% VS （23.67±2.85）%. Conclusion：The discrepancy of detrusor contraction and M3 muscarinic receptors in diabetic cystopathy （DCP） is the marker of affection aggravation. It makes the function for further study of the merchanisms of DCP.

关 键 词：糖尿病膀胱病 逼尿肌 M3受体 膀胱重构 

分 类 号：R587.1[医药卫生—内分泌]