血管肽酶抑制剂对大鼠心梗后心室重构和胚胎基因表达的影响  

Effects of Vasopeptidase Inhibitor on the Expression of Myocardium Fetal Gene After Myocardial Infarction in Rats

作　　者：邹军[1] 黄景文[1] 罗韶金[1] 杨玲[1] 杨泽福[1] 薛世荣[1] 王江华[1] 

机构地区：[1]南方医科大学附属南海医院心内科,广东佛山528200

出　　处：《武汉大学学报（医学版）》2013年第4期494-498,共5页Medical Journal of Wuhan University

基　　金：广东省佛山市医学类科技攻关项目(编号:201208163)

摘　　要：目的:探讨血管肽酶抑制剂(VPI)Omapatrilat对大鼠心肌梗死后心肌细胞胚胎基因(αMHC、βMHC、SKACT)的表达以及对心室重构和心功能的影响。方法:结扎大鼠冠状动脉前降支建立心肌梗死(MI)模型,随机分为假手术组(Sham组)、心肌梗死组(MI组)和VPI(Omapatrilat)干预组(MI+VPI组)。术后24hMI+VPI组大鼠给予Omapatrilat40mg/(kg·d),Sham组和MI组饲普通饮水。术后4周压力传感器记录左室血流动力学改变;Masson氏染色测心肌胶原容积分数(CVF)及心肌血管周围胶原面积(PVCA);逆转录聚合酶链(RT-PCR)检测心肌组织胚胎基因的表达。结果:与Sham组比较,MI组αMHC mRNA表达明显降低,βMHC、SKACT mRNA表达显著上调,非梗死区胶原沉积及全心重量指数、左室相对重量增加;VPI药物干预组上调MI后受抑制的αMHCmRNA表达及减少βMHC、SKACT mRNA表达,且非梗死区胶原沉积及全心重量指数、左室相对重量均较MI组明显降低。结论:Omapatrilat抑制大鼠心肌梗死后的心室重构,改善心功能,其作用可能与调节胚胎基因的表达有关。Objective. To investigate the effects of vasopeptidase inhibitor（VPI） on ventricular remode- ling, cardiac function, and expression of myocardium fetal genes after myocardial infarction （MI） in rats. Methods. Male SD rats were randomly divided into sham, MI, and MI＋VPI（omapatri- lat） groups. Myocardial infarction was produced by ligation of the left anterior descending coro- nary artery. After 24 hours of operation, rats in the MI ＋ VPI group were treated with 40 mg/ （kg · d） Omapatrilat in their drinking water, and rats in the sham and MI groups were treated with placebo. Four weeks after MI, the left ventricular hemodynamics was recorded with pres- sure transducer, and collagen volume fraction （CVF） and perivascular collagen volume area （PV- CA） were measured with Masson staining. The fetal gene expression of myocardium fetal gene were determined with RT-PCR. Results. Four weeks after MI, the expression of aMHC mRNA of MI group were significantly decreased, and the βMHC and SKACT mRNA raised when cornpared with that of Sham group. The total heart weight index and relative weight of the left ven- tricle increased. The expression of the suppressed aMHC mRNA was upreguated, while that of βMHC and SKACT mRNA decreased by VPI after MI. The total heart weight index and the rela- tive weight of the left ventricle in the groups of VPI lowered significantly. Conclusion： The va- sopeptidase inhibitor shows favorable effects on left ventricular remodeling after MI in rats and improves cardiac function. These effects may contribute to the regulation of myocardium fetal gene expression in left ventricular.

关 键 词：血管肽酶抑制剂 心室重构 胚胎基因 心肌梗死 

分 类 号：R331.31[医药卫生—人体生理学]