盐酸戊乙奎醚对脓毒症小鼠肺血管内皮细胞黏附分子-1和髓过氧化物酶影响  

Effects of Penehyclidine Hydrochloride on Lung Vascular Cell Adhesion Molecule-1 and Myeloperoxidase Levels in Septic Mice

作　　者：詹佳[1] 肖飞[2] 李进杰[1] 王焱林[1] 王轶芃[1] 张宗泽[1] 

机构地区：[1]武汉大学中南医院麻醉科湖,北武汉430071 [2]华中科技大学附属普爱医院骨科,湖北武汉430033

出　　处：《武汉大学学报（医学版）》2013年第4期504-506,532,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金青年基金项目(编号:81101408);中央高校基本科研业务费专项资金资助项目(编号:111169)

摘　　要：目的:探讨盐酸戊乙奎醚(PHC)对脓毒症小鼠肺组织血管内皮细胞黏附分子-1(VCAM-1)水平、髓过氧化物酶(MPO)活性和β抑制蛋白-1(β-arrestin-1)表达的影响。方法:建立脓毒症小鼠模型,实验动物随机分为假手术组、盲肠结扎穿孔模型组(CLP组)和盐酸戊乙奎醚组(PHC组),每组10只。各组小鼠于造模12h时间点采血检测血乳酸水平,收集肺组织检测VCAM-1水平、MPO活性和β-arrestin-1mRNA的表达。结果:与假手术组比较,CLP组血乳酸值、肺VCAM-1水平和MPO活性升高,β-arrestin-1mRNA表达降低;与CLP组比较,PHC组可降低血乳酸值、肺VCAM-1水平和MPO活性,而增加β-arrestin-1mRNA表达。结论:盐酸戊乙奎醚预处理,可以通过上调β-arrestin-1的表达,从而降低VCAM-1水平和MPO活性,减轻脓毒症小鼠肺损伤。Objective： To investigate the effects of penehyclidine hydrochloride （PHC） on lung vascular cell adhesion molecule-1 （VCAM-1） level, myeloperoxidase （MPO） activity, and β-arrestin-1 mRNA level in septic mice. Methods： Mice were randomly divided into three groups （n= 10 each） ： sham operation group, cecal ligation and puncture group （CLP group） and penehyclidine hydrochloride 0.45 mg/kg group （PHC group）. Blood sample was collected to measure serum lactic acid （LD）. The lung tissue samples were collected to examine VCAM-1 level, MPO activi- ty, and expression of β-arrestin-1 mRNA at the 12th hour after surgery. Results： Compared with that respectively in sham group, serum LD, lung VCAM-1 level and MPO activity increased sig- nificantly, while expression of β-arrestin-1 decreased in CLP group. Furthermore, compared with that respectively in CLP group, PHC group markedly decreased serum LD, lung VCAM-1 level, and MPO activity, and increased expression of β-arrestin-1 mRNA. Conclusion： PHC could up regulate the expression of β-arrestin-1, reduce VCAM-1 level and MPO activity, and ameliorate the acute lung injury of septic mice.

关 键 词：盐酸戊乙奎醚 血管内皮细胞黏附分子-1 髓过氧化物酶 β抑制蛋白-1 脓毒症 

分 类 号：R631[医药卫生—外科学]