秋水仙素诱导不结球白菜叶片形态类型与染色体加倍的关系  

Relationship between Leaf Morphological Type and Chromosome Doubling of Non-heading Chinese Cabbage Induced by Colchicine

作　　者：徐丽娟[1] 张蜀宁[1] 郑于莉 张宇[1] 侯喜林[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,南方蔬菜遗传改良重点开放实验室,南京210095

出　　处：《西北植物学报》2013年第6期1239-1244,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：江苏省科技支撑项目(BE2011313)

摘　　要：以二倍体不结球白菜‘依伶’为材料,用0.2%秋水仙素溶液处理子叶期生长点,研究不结球白菜在形态学上对秋水仙素诱导的响应及动态变化。结果表明:(1)‘依伶’对秋水仙素诱导的响应均以幼苗期前4片真叶中出现变异特征叶的形式表现,诱导处理15d后出现了圆盘状、豆瓣状、棒状3种响应类型;处理30d后,以上3种类型分别分化出长勺状、花状、圆锥状类型。(2)分化前后3种响应类型受秋水仙素抑制程度不同导致生长速率各不相同,生长速率的顺序分别为:圆盘状>豆瓣状>棒状,花状>长勺状>圆锥状,6种响应类型的特征叶逐渐萎蔫死亡,继续生长的其他.叶生长正常。(3)豆瓣状、圆盘状、长勺状、棒状、花状、圆锥状6种叶片形态响应类型于处理当代选株中四倍体加倍率依次分别为50.00%、41.67%、25.00%、16.67%、8.33%、0.00%。研究表明,早期筛选宜选用圆盘状、豆瓣状变异植株。With diploid non-heading chinese cabbage‘Yiling’as material,0.2% colchicines was dropped in growth point of cotyledon stage.We studied non-heading Chinese cabbage response on colchicine induced and dynamic change in morphology.The results showed that：（1）‘Yiling’response on colchicine induced is that variation of leaf appear in the first four leaves of seedling stage.After 15 days of treatment,we observed three response types：disc character,bean character and coliform character.After 30 days of treatment,disc character,bean character and coliform character is developed macro-scoop character,flower character and conical character respectively.（2）The above six response types have different degrees of inhibition of seedling stage：disc characterbean charactercoliform character,flower charactermacro-scoop characterconical character;the variation characteristic leaf wilting and died gradually,other variation leaves grow normally.（3）disc character,bean character,coliform character,macro-scoop character,flower character and conical character,Rate of tetraploid of six responsed types above is 50.00%,41.67%,25.00%,16.67%,8.33%and 0.00%,repectively,disc character and bean character should be used for early screening.

关 键 词：不结球白菜 秋水仙素 响应 形态学 动态变化 

分 类 号：Q813.5[生物学—生物工程]