背侧抑制性轴突导向蛋白抑制鸡胚脊髓神经嵴细胞的迁移  

Draxin inhibits the migration of chick neural crest cells

作　　者：耿丹丹[1] 崔慧先[1] 张三兵[2] 王磊[1] 康林[1] 苏玉红[1] 

机构地区：[1]河北医科大学人体解剖学教研室,石家庄050017 [2]石家庄市第三医院手足外科,石家庄050011

出　　处：《神经解剖学杂志》2013年第4期355-359,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31100789);教育部高等学校博士学科点专项科研基金(20111323120006);河北省自然科学基金(C2011206032)

摘　　要：目的:初步探讨背侧抑制性轴突导向蛋白(draxin)在鸡胚脊髓神经嵴细胞迁移过程中的作用。方法:应用神经嵴细胞特异性标记物HNK-1免疫组化染色检测不同发育阶段正常鸡全胚脊髓神经嵴细胞迁移特性;鸡draxin表达载体质粒转染COS7细胞株,收集上清作为条件培养液。分别用含draxin的条件培养液处理体外培养的鸡全胚和鸡胚脊髓神经管,观察在体和离体条件下draxin对脊髓神经嵴细胞迁移特性的影响。结果:HH12-13到HH18-19阶段正常鸡胚脊髓发育过程中,神经嵴形成明显的从头侧到尾侧的渐进性、节段性迁移特性;draxin对体外培养的全胚和神经管内神经嵴细胞的迁移均具有明显抑制性调控作用。结论:Draxin抑制早期鸡胚脊髓神经嵴细胞的迁移。Objective： To investigate the role of a repulsive axon guidance protein, draxin, in the migration of chick neural crest cells. Methods： Immunohistochemical staining of HNK-1, a neural crest cells＇ marker, was used to observe the migration pathway of neural crest cells at different embryonic stages. COS7 cells were transfected with chick draxin cDNA for 4 days and the supernatant was harvested as conditioned medium （CM）. Then the effects of draxin CM in chick whole-embryo and neural tube culture were observed in vitro. Results： At different embryonic stages, chick neural crest migration was anterior-posterior gradient in chick embryonic spinal cord. Draxin significantly reduced the migration of neural crest cells in both chick whole-embryo and neural tube cultures. Conclusion： Draxin was a repulsive regulator for chick neural crest cells migration.

关 键 词：Draxin 神经嵴 细胞迁移 条件培养液 鸡全胚培养 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]