前列腺环素E2在体外通过Wnt/β-catenin信号刺激神经元发生  

Prostaglandin E2 stimulates neurogensis through β-catenin signaling in vitro

作　　者：杨佳蕾[1] 杨振宇[1] 樊洪[1] 吴寅[1] 王亚周[1] 

机构地区：[1]第四军医大学基础部神经生物学教研室,西安710032

出　　处：《神经解剖学杂志》2013年第4期379-385,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31271583)

摘　　要：目的:在体外探讨前列腺环素E2对神经干细胞增殖分化的影响及可能的分子机制。方法:原代培养小鼠胚胎神经干细胞。分为溶剂对照组;100 nmol/L、1μmol/L前列腺环素E2处理组;1μmol/L前列腺环素E2加15μmol/L XAV939处理组。上述各组细胞在无血清增殖培养基中培养两天后,行BrdU、PY489染色,观察神经干细胞的增殖及Wnt信号的激活;在含2%胎牛血清的分化培养基中培养四天后,行Tuj/GFAP,CNPase染色,观察神经干细胞的分化。结果:100 nmol/L,1μmol/L前列腺环素E2可分别使神经干细胞的增殖增加约86%和195%,及其向神经元方向的分化增加约85%和257%,抑制向星形胶质细胞方向的分化,但不影响向少突胶质细胞方向的分化。100 nmol/L,1μmol/L前列腺环素E2可使核型β-catenin阳性细胞的百分率分别增加约31%和91%。15μmol/L XAV939可显著降低1μmol/L前列腺环素E2诱导的神经干细胞增殖及其向神经元方向的分化。结论:前列腺环素E2可在体外通过Wnt/β-catenin信号促进神经干细胞增殖,并诱导其向神经元方向分化。Objective： To assess the effects of prostaglandin E2 （PGE2） on the proliferation and differentiation of neu- ral stem cells, and the corresponding mechanisms in vitro. Methods： Primary neural stem cells were treated with （ 1 ） ve- hicle control; （2） 100 nmol/L, 1 μmol/L PGE2; （3） 1 μmol/L PGE2 plus 15 μmol/L XAV939. After 2d＇s culture in proliferative medi μmol/L, immunostaining of BrdU and PY489 were performed to assess cell proliferation and Wnt signa- ling activation. After 4d＇s culture in the differentiating medium, immunostaining of GFAP, Tujl and CNPase were con- ducted to evaluate the differentiation of neural stem cells. Results： 100 nmol/L and 1 μmol/L PGE2 significantly pro- mote the proliferation of neural stem cells by about 86% and 195%, and neuronal differentiation by 85% and 257%, suppress the astrocytic differentiation of neural stem cells, but not the generation of oligodendrocyte. 100 nmol/L and 1 μmol/L PGE2 significantly increase the percentages of nuclear β-catenin by 31% and 91%. Wnt signaling inhibitor, XAV939 can significantly decrease the PGE2-induced proliferation and neuronal differentiation of neural stem cells. Con- clusion： PGE2 can stimulate neurogenesis through Wnt/β-catenin signaling in vitro.

关 键 词：神经干细胞 增殖 分化 前列腺环素E2 WNT β-catenin信号 

分 类 号：R338[医药卫生—人体生理学]