小鼠脊髓灰质发育过程中细胞迁移与血管之间的关系  

The relafionship between cell migration and vasculature in the developing spinal cord of mouse

作　　者：王志新[1] 王卉[1] 王延芬[2] 高晓群[1] 

机构地区：[1]郑州大学基础医学院人体解剖学教研室 [2]郑州大学医学院脑瘫研究中心,郑州450000

出　　处：《神经解剖学杂志》2013年第4期415-420,共6页Chinese Journal of Neuroanatomy

基　　金：河南省科技厅攻关项目(122102310205)

摘　　要：目的:探讨小鼠神经系统发育过程中脊髓成熟神经元迁移与血管发育之间的关系。方法:不同年龄小鼠共计75只,应用免疫荧光及墨汁灌注的技术,标记小鼠胚胎E17到P30脊髓神经元和血管。结果:大约在胚胎E17左右,小鼠脊髓灰质内开始出现NeuN阳性的神经元,白质中神经元较少,且此时灰质和白质内血管分布均匀,管径一致,分支较少。随着年龄的增长,脊髓周围的神经元不断向内迁移,灰质内NeuN阳性的神经元数先增多后减少且血管体密度先增加后减小,而白质内的神经元持续减少,血管逐渐稀疏。P14以后,脊髓灰质内的血管密度明显高于白质内的血管密度。同时,研究中还发现部分NeuN阳性细胞紧贴着血管分布。结论:在小鼠脊髓的发育过程中,NeuN阳性细胞由脊髓周围逐渐向中心迁移,和脊髓灰质H形态的形成密切相关。同时,血管扮演着重要的角色,它可能通过与神经元的相互作用,引导神经元的迁移,并且为神经元的迁移提供路径和支架。Objective： To explore the interaction between angiogenesis and neural migration in developing spinal cord of mice. Methods： A total of 75 mice aged variously were wsed the immunofluorescence and ink perfusion were used to la- bel the neurons and vasculature in the mouse spinal cord aged from El7 （embryonic day 17 ） to P30 （postnatal day 30）. Results ： NeuN-positive neurons began to appear in the grey matter of spinal cord at about El7, but there were fewer neu- rons in white matter. In the meantime, in grey matter and white mater vascular network distribution was uniform, and lu- mens were consistent with less branches. With age increasing, NeuN-positive neurons migrated to the center from around. Both the NeuN-positive neurons and vascular density in grey matter increased and then decreased, but positive neurons and vascular density in white matter always decreased. After P14 vascular density in grey matter was much more than that in white matter. Finally, the study also showed that some NeuN-positive cells could migrate along blood vessels. Conclu- sion ： NeuN-positive neurons migrated to the center from the around in developing spinal cord of mice, which was closely related to the formation of H shape grey matter. And blood vessels played important role in suiing the migration of neurons in the spinal cord, probably serving as scaffolds for the neuronal migration.

关 键 词：脊髓 神经元 血管 免疫组化 

分 类 号：R338[医药卫生—人体生理学]