苏木对饥饿期粪肠球菌生物膜作用的体外研究  

The effect of herbal Sapanwood on the biofilm of Entercoccus faecalis in starvation phase

作　　者：许颖[1] 于博文[1] 康梁[1] 张慧明[2] 范淑霞[3] 

机构地区：[1]佳木斯大学附属口腔医院牙体牙髓病二科,黑龙江佳木斯154007 [2]佳木斯大学生命科学实验中心,黑龙江佳木斯154007 [3]齐齐哈尔市第一医院精神五官分院口内科,黑龙江齐齐哈尔161005

出　　处：《中国微生态学杂志》2013年第7期810-812,共3页Chinese Journal of Microecology

摘　　要：目的验证中药苏木对饥饿期粪肠球菌生物膜的抑制作用。方法建立饥饿期粪肠球菌生物膜体外模型,MTT法计算中药苏木对饥饿期粪肠球菌生物膜的抑菌率。并利用激光共聚焦显微镜(CLSM)观察药物对生物膜中粪肠球菌的影响。结果中药苏木对饥饿期粪肠球菌生物膜的抑制作用随药物浓度增加而增加。CLSM观察见中药苏木可使饥饿期粪肠球菌生物膜内活菌比例明显下降。结论中药苏木对饥饿期粪肠球菌生物膜具有一定抑制作用。Objective To confirm the inhibition of herbal Sapanwood to the biofilm of Entercoccus faecalis（E.faecalis） in starvation phase.Methods The biofilm models of E.faecalis in the starvation phase were built in vitro;the inhibition rate of Sapanwood to the biofilms of E.faecalis in starvation phase were calculated MTT;Confocal laser scanning microscopy（CLSM） was used to observe the effect of sapanwood on E.faecalis in biofilms.Results The inhibitory effect of herbal Sapanwood on the biofilms of E.faecalis in starvation phase increased along with the drug concentration.CLSM result indicated that the ratio of viable bacteria in the biofilm was reduced.Conclusion Sapanwood has an obvious inhibitory effect on the biofilm of E.faecalis in the tarvation phase.

关 键 词：饥饿 粪肠球菌 苏木 生物膜 

分 类 号：R378.1[医药卫生—病原生物学]