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作 者:赵彩霞[1] 王丽萍[1] 孙万仓[1] 武军艳[1] 刘自刚[1] 何丽[1] 李林蔚[1]
机构地区:[1]甘肃省干旱生境作物学重点实验室,甘肃农业大学,甘肃兰州730070
出 处:《干旱地区农业研究》2013年第4期129-134,共6页Agricultural Research in the Arid Areas
基 金:农业部公益性行业(农业)科研专项(200903002-04);国家高技术研究发展计划("863"计划)项目(2011AA10A104);现代农业产业技术体系建设专项(CARS-13)
摘 要:以白菜型冬油菜品种鉴94为材料,研究化学药物GSC的不同浓度对白菜型冬油菜主要性状的影响结果表明,GSC处理两次后,导致植株生长势变弱,而对结实力无显著影响;对油菜花冠和花瓣的生长有一定抑作用,随着化学药物浓度的增加,花冠直径和花瓣面积呈减小趋势,雄蕊长度逐渐缩短,雌蕊无显著变化,T7浓度理(第一次0.5mg·L-1和第二次0.6mg·L-1浓度处理)后,花丝萎缩,花药干瘪,出现大量不育花粉。GSC可以诱白菜型油菜产生雄性不育,随着GSC浓度的增加,不育株数和失活花粉数也逐渐增加,在T7浓度,植株不育株率86.49%,失活花粉率达89.30%;同时用此浓度处理后花粉囊发育不良,绒毡层细胞提前解体或随花粉粒退化一消失,小孢子团紧贴内壁,数量变少。试验说明化学药物GSC对白菜型冬油菜具有一定的杀雄效果,同时杀雄效与药物浓度有一定关系。Using the cuhivar Jian 94 as a material, the effects of different concentrations of the chemical agent GSC on the main traits of winter rapeseed ( Brassica rapa ) were studied. The results showed: GSC treatment caused a negative effect on plant growth, but there was no significant effect on seed setting; the growth of corolla and petals was inhibited, and the corolla diameter and petal area were decreased with the increase of GSC concentration; the length of stamens was gradually shortened, while the pistils were not changed significantly; under the concentration of T7 (0.5 mg·L-1 and 0.6 mg·L-1 at first and second time, respectively), the filaments became atrophied, and the anthers became shriveled with a large number of sterile pollens. GSC could induce male sterility of winter rapeseed, and the rate of sterile plants and dead pollens was increased with the rise of GSC concentration. Under the concentration of TT, the rate of sterile plants and dead pollens was 85.67% and 82.30%, respectively. Meanwhile, the anthers became hypogenetic, the tapetal cells disintegrated in advance or disappeared along with the degradation of pollen grains, and the spore balls stuck closely to the inner wall and their number became small. The results demonstrated that the chemical agent GSC could cause a certain effect on male sterility, and there was a relationship between sterilization rate and GSC concentration.
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