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作 者:汪军成[1,2] 孟亚雄[1,2] 徐先良[1,2] 王晋[1,2] 赖勇[1,2] 李葆春[3] 马小乐[2] 王化俊[1,2]
机构地区:[1]甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070
出 处:《干旱地区农业研究》2013年第4期135-143,共9页Agricultural Research in the Arid Areas
基 金:国家支撑计划项目(2007BAD52B08);国家自然基金项目(30771331);大麦青稞产业技术体系岗位(CARS-XX)
摘 要:为了了解20份大麦(HordeumvulgareL.)种质材料抗旱性差异,采用温室模拟干旱胁迫-复水法,对其苗期的相对含水量、相对电导率、叶绿素含量、丙二醛含量、超氧化物歧化酶和株高等8个抗旱指标进行测定,利用相关分析、聚类分析研究了各项指标与种质材料抗旱性关系,综合评价其抗旱性。结果显示:叶片相对含水量与株高呈显著正相关,其余各指标间没有显著的相关性;以采用欧式距离法综合分析结果为依据,可将20份种质材料划分为3个抗旱等级,相对抗旱包括陇麦1号、新引D5、Z126V004W、新引D9、PREJTGE、SCARLETT、Z02751407、韭崎2号、Z127U016V、MAURITIA,中等抗旱包括J13-80、新引D7、PASADENA、NCF-TIPPLE、甘啤6号,相对敏感包括黑引瑞、Z200V017W、新啤12号、BEATRIX、Z204V021W。With the method of drought stress and water recovery simulated in greenhouse, 8 drought resistant indicators, including leaf relative water content (RWC), relative electrical conductivity (REC), chlorophyll content (Chl), malondialdehyde content (MDA), soluble sugar content (SSC), free proline accumulation (Pro), superoxide dismutase activity (SOD) and plant height (PH) were measured for 20 germplasms of Hordeum vulgate L., and the relationship between these indicators and drought resistance was determined by correlation analysis and cluster analysis, so as to eval- uate the drought resistance of different germplasms. The results showed that the RWC was significantly and positively correlated with the plant height, and the tested germplasms of Hordeum vulgate L. could be classified into three groups: in the high drought-resistance group, there were Lngrnai 1, Xinyin DS, Z126VOO4W, Xinyin D9, PREJTGE, SCAR-LETF, Z02751407, Jiuqi 12, Z127U016V and MAURITIA; in the medium drought-resistance group, there were J13 - 80, Xinyin D7, PASADENA, NCF-TIPPLE and Ganpi 6; and in the low drought-resistance group, there were Heiyin- rui, Z200V017W, Xinpi 12, BEATRIX and Z204V021W.
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