锰抗性微生物的筛选鉴定及其对Mn^(2+)的吸附  

Screening and Identification of a Manganese-resistant Strain and Its Manganese Biosorption Conditions

作　　者：王燕[1] 何峰[2] 尤健健[2] 陈坤[3] 徐冰沁[3] 林雁冰[2] 

机构地区：[1]杨凌职业技术学院,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100 [3]西北农林科技大学创新学院,陕西杨凌712100

出　　处：《西北农业学报》2013年第7期193-199,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31270530);校内博士科研启动项目(2011BSJJ067);中央高校基本科研业务费专项资金(QN2011025)

摘　　要：采用平板分离法从陕西南宝锰矿尾矿土样中分离的放线菌中筛选出一株高效抗锰菌株NB-7,抗锰最高质量浓度达40mg/L。通过形态和16SrRNA基因序列分析对该菌株进行鉴定,并综合运用单因素和正交试验对该菌株吸附锰的条件进行优化。结果表明,菌株NB-7为刺疣链霉菌(Streptomycesspinoverrucosus)。初始pH、接种量、初始锰离子质量浓度及吸附时间对锰离子的生物吸附有较显著影响,而吸附温度基本无影响。各因素对吸附率的影响表现为接种量>初始锰离子质量浓度>初始pH>吸附时间;正交试验最终确定菌株NB-7吸附锰离子的最佳条件为:初始pH为4,接种量4.0%,吸附时间30min,初始锰离子质量浓度20mg/L。菌株NB-7在最佳条件下对锰离子的生物吸附具有较好的重复性和稳定性,最高吸附量在85%左右。Strain NB-7 was isolated from soil samples which were collected from an electrolytic manga- nese processing factory of Shaanxi Nanbao Manganese Industry. The strain NB-7 was resistant to the maximum Mn2＋ mass concentration 40 mg/L. Morphological and 16S rRNA gene sequence were used for identification. Strain NB-7 was identified as Streptomyces spinoverrucosus. Both single factor and orthogonal experiments were designed to determine the optimum conditions for manganese biosorp- tion. The result showed that inoculation dose, initial pH, initial Mn2＋ mass concentration and adsorption time had significant impacts on the biosorption process while temperature showed little influence. The effect of single factor order showed inoculation dose〉initial Mn2＋ mass eoncentration〉 initial pH〉adsorption time. The optimum conditions for manganese biosorption were initial pH 4, inoculation dose 4.0%, adsorption time 30 min, initial Mn2＋ mass concentration 20 mg/L. The strain grew well with good repeatability and stability under the optimum conditions, and the maximum biosorption capacity of NB-7 under the experimental conditions reached more than 85 %0. This research laid the foundation for further study on actinomyces adsorption mechanism of manganese and its application in bioremediation.

关 键 词：刺疣链霉菌 锰 吸附 单因素 正交 

分 类 号：X703[环境科学与工程—环境工程]