素馨花总环烯醚萜苷的大孔树脂富集纯化工艺优选  

Optimization of Enrichment and Purification Technology for Total Iridoid Glycosides from Jasminum officinale by Macroporous Resin

作　　者：罗赛赛[1] 郝婷[1] 赵桂琴[1] 

机构地区：[1]承德医学院中药研究所,河北省中药研究与开发重点实验室,河北承德067000

出　　处：《中国实验方剂学杂志》2013年第15期13-16,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省自然科学基金项目(C2010001354)

摘　　要：目的:优选大孔树脂富集纯化素馨花总环烯醚萜苷的工艺条件。方法:采用HPLC测定橄榄苦苷含量。以橄榄苦苷为指标成分,通过静态吸附-洗脱试验筛选大孔树脂型号,通过单因素试验和正交试验考察大孔树脂富集纯化工艺。结果:XDA-16型大孔树脂吸附洗脱性能最好,最佳纯化工艺条件为上样液生药质量浓度0.08 g.mL-1,上样量0.7 g.g-1,树脂柱径高比1∶3,吸附流速2 BV.h-1,加8 BV水洗除杂,用70%乙醇4 BV于2 BV.h-1流速洗脱,收集洗脱液。结论:该工艺条件稳定可行,XDA-16型大孔树脂可有效地富集纯化素馨花总环烯醚萜苷。Objective： To optimize enrichment and purification technology of total iridoid glycosides from Jasminum officinale by macroporous resin.Method： With oleuropein as index,its content was determined by HPLC.Types of macroporous resin were optimized by static adsorption-elution test,enrichment and purification technology was investigated by orthogonal test and single-factor tests.Result： XDA-16 macroporous resin showed better effect than others,optimum purification technology was as following： the concentration of sample liquid 0.08 g.mL-1,height-diameter ratio of 1∶ 3,absorption velocity 2 BV.h-1,dosage of sample liquid of 0.7 g.g-1,washed impurity with 8 BV water,then eluted wtih 4 BV 70% ethanol at 2 BV.h-1,collected eluent.Conclusion： This optimized technology was stable and feasible.XDA-16 macroporous resin had a good enrichment and purification effect on total iridoid glycosides from J.officinale.

关 键 词：素馨花 总环烯醚萜苷 大孔树脂 正交试验 橄榄苦苷 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]