电导率法在线监测栀子提取物大孔树脂柱分离工艺  

Online Monitoring of Separation Technology with Macroporous Resin Column for Extract of Gardenia jasminoides by Conductivity Method

作　　者：张鹏[1] 高峻峰[1] 孟硕[1] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《中国实验方剂学杂志》2013年第15期27-28,共2页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30772755;81073060);国家科技重大专项(2009ZX09103-317)

摘　　要：目的:利用电导法在线监测栀子提取液大孔树脂柱分离工艺。方法:利用电导计测定通过大孔树脂柱洗脱后流出成分的电导率变化,并考察其与去乙酰基车叶草苷酸甲酯、京尼平-1-β-D龙胆双糖苷、栀子苷3个成分变化的相关性。结果:电导率先下降,之后随栀子等成分的被洗脱又明显升高,呈较好的相关性。结论:电导法可用于监测栀子提取物的大孔树脂分离工艺。Objective： To investigate online monitoring of separation technology with macroporous resin column for extract of Gardenia jasminoides by conductivity method.Method： Conductivity changes of eluted components after macroporous resin absorption were determined by conductivity apparatus,and its correlation with changes of geniposide,genipin-1-β-D-gentiobioside and deacetyl-asperulosidic acid methyl ester was detremined respectively.Result： Conductivity decreased first,and then significantly increased with elution of geniposide and other components,showing a good correlation.Conclusion： Conductance method could be used to monitor separation technology with macroporous resin for extract of G.jasminoides.

关 键 词：大孔树脂 栀子 电导率 在线监测 去乙酰基车叶草苷酸甲酯 京尼平-1-β-D龙胆双糖苷 栀子苷 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]