紫草总萘醌的闪式提取工艺优选  

Optimization of Smashing Tissue Extraction Technology of Total Naphthaquinones in Arnebia euchroma

作　　者：曾祖平[1] 钱珊[2] 王宏[1] 彭冰[1] 韩旭阳[1] 李萍[1] 

机构地区：[1]首都医科大学附属北京中医医院北京市中医研究所,北京100010 [2]北京中医药大学东方学院,河北廊坊065001

出　　处：《中国实验方剂学杂志》2013年第15期29-31,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市中医药科技提升专项(KJTS2011-07)

摘　　要：目的:优选紫草中总萘醌的闪式提取工艺。方法:以紫草总萘醌和β,β'-二甲基丙烯酰阿卡宁转移率为指标,采用L9(34)正交试验考察溶剂用量、提取时间、提取电压对闪式提取工艺的影响,并与渗漉法进行比较。结果:最佳提取工艺为分别加14,8倍量95%乙醇于140 V电压下提取2次,每次2 min;总萘醌和β,β'-二甲基丙烯酰阿卡宁平均转移率分别为90.72%,70.68%。渗漉法中总萘醌和β,β'-二甲基丙烯酰阿卡宁平均转移率分别为79.03%,65.43%。结论:闪式提取法优于渗漉法,前者适用于紫草总萘醌的提取,优选的工艺稳定可行,具有良好的应用前景。Objective： To optimize smashing tissue extraction technology of total naphthaquinones in Arnebia euchroma.Method： With transfer rates of total naphthaquinones and β,β’-dimethylacrylalkannin as evaluation indexes,L9（34） orthogonal design was adopted for investigating effect of the amount of solvent,extraction time and voltage on smashing tissue extraction technology,and compared with percolation method.Result： Optimum extraction conditions were as follows： extracted 2 times with 14,8 folds the amount of 95% ethanol at 140 V,2 minutes per time.Transfer rates of total naphthaquinones and β,β’-dimethylacrylalkannin were 90.72% and 70.68%,but they were 79.03% and 65.43% with percolation method,respectively.Conclusion： Smashing tissue extraction method was better than percolation method,the former was suitable for extracting total naphthaquinones in A.euchroma,this optimized technology was stable and feasible with good application prospects.

关 键 词：紫草 闪式提取 正交试验 紫外-可见分光光度 高效液相色谱 紫草总萘醌 β β'-二甲基丙烯酰阿卡宁 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]