芫花酯甲的快速TLC纯化技术与鉴定  

Rapid TLC Purification and Identification of Yuanhuacine

作　　者：王玲[1] 李娆娆[2] 孙婷婷[2] 刘延泽[3] 

机构地区：[1]河南中医学院药学院,郑州450008 [2]中国中医科学院中药研究所,北京100700 [3]中国医学科学院药用植物研究所,北京100193

出　　处：《中国实验方剂学杂志》2013年第15期41-43,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京自然科学基金项目(7112097)

摘　　要：目的:制备高纯度、低成本的芫花酯甲对照品。方法:采用硅胶柱-制备TLC制备芫花酯甲单体,通过HPLC分析纯度,NMR确定结构。结果:硅胶柱-制备TLC比制备液相成本低,未发现可见杂质斑点,所得单体纯度较好。结论:该方法解决了本品在分离纯化方面高消耗低产出的难题,为芫花萜类化合物的研究与开发提供参考。Objective： To prepare yuanhuacine reference with high purity and low cost.Method： Yuanhuacine was obtained by Silica gel column-preparative TLC method.Purity of yuanhuacine was determined by RP-HPLC and structure was identified by NMR.Result： Cost of silica gel column-preparative TLC method was lower than preparative HPLC,no visible impurity spot,purity of prepared yuanhuacine was good.Conclusion： Problem of high cost,low yield of yuanhuacine in preparation could be resolved by this method,it provided a reference for research and development of terpenoids in Daphne genkwa.

关 键 词：芫花酯甲 制备TLC 纯化技术 

分 类 号：R284.2[医药卫生—中药学] R284.1[医药卫生—中医学]