HPLC测定穿心莲配伍绵茵陈后2种内酯成分含量的变化  

Determination of Content Change of Two Lactone Ingredients from Andrographis Herba after Compatibility with Artemisiae Scopariae Herba by HPLC

作　　者：陈倩倩[1] 李清[1] 齐会红[1] 李玉娇[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学中药学院,中药质量控制技术国家地方联合工程实验室,沈阳110016

出　　处：《中国实验方剂学杂志》2013年第15期64-66,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2012年辽宁省高等学校中药质量控制关键技术创新团队项目(LT2012018)

摘　　要：目的:考察穿心莲与绵茵陈配伍后对穿心莲内酯和脱水穿心莲内酯含量变化的影响。方法:采用RP-HPLC,Innersil ODS-3色谱柱(4.6 mm×250 mm,5.0μm),流动相乙腈-水梯度洗脱,流速1.2 mL.min-1,波长检测程序(0～10 min,225 nm;10～20 min,254 nm),柱温35℃。结果:穿心莲内酯和脱水穿心莲内酯质量浓度分别在19.66～314.6(r=0.999 5),4.32～69.12 g.L-1(r=0.999 5)与峰面积呈良好线性关系,平均加样回收率分别为99.7%,99.9%,RSD均<2.3%。结论:穿心莲与绵茵陈配伍后水煎液中2种内酯类成分含量显著增加。Objective： To investigate effects of Andrographis Herba compatibility with Artemisiae Scopariae Herba on content change of andrographolide and dehydroandrographolide.Method： RP-HPLC was performed on an Innersil ODS-3 column（4.6 mm × 250 mm,5.0 μm） with mobile phase of acetonitrile-water in gradient elution,flow rate was 1.2 mL.min-1,detection wavelengths were set at 225 nm for andrographolide in 0-10 min and 254 nm for dehydroandrographolide in 10-20 min,column temperature was 35 ℃.Result： The linear range of andrographolide and dehydroandrographolide were 19.66-314.6（r = 0.999 5）,4.3-69.12 g.L-1（r = 0.999 5）,respectively.Average recoveries of these two components were 99.7% and 99.9%,respectively with RSD were less than 2.3%.Conclusion： The contents of andrographolide and dehydroandrographolide from Andrographis Herba increased significantly after compatibility with Artemisiae Scopariae Herba.

关 键 词：穿心莲 绵茵陈 配伍 含量变化 穿心莲内酯 脱水穿心莲内酯 

分 类 号：R284.1[医药卫生—中药学]