HPLC测定狗皮膏中桂皮醛和丁香酚的含量  

Content Determination of Cinnamaldehyde and Eugenol in Goupi Gao by HPLC

作　　者：王森[1] 陈爱华[1] 刘红宁[1] 朱卫丰[1] 李荣苗[1] 黄招光[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2013年第15期73-75,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十一五"科技支撑计划项目(2008BA153B071)

摘　　要：目的:建立HPLC测定狗皮膏中丁香酚和桂皮醛含量的方法。方法:Phenomenex-Luna C18色谱柱(4.6 mm×250mm,5μm),流动相乙腈-0.1%磷酸(45∶55),流速1.0 mL.min-1,柱温40℃,检测波长280 nm。结果:桂皮醛在0.141 69～14.169 mg.L-1,丁香酚在1.066 04～106.604 mg.L-1质量浓度与峰面积均呈良好的线性关系;桂皮醛和丁香酚的平均回收率分别为102.98%(RSD 1.33%),95.40%(RSD 0.40%)。结论:该方法简便,快速,准确,重复性好,可用于狗皮膏中丁香酚和桂皮醛的含量测定。Objective： To establish an HPLC method for the determination of cinnamaldehyde and eugenol in Goupi Gao.Method： The HPLC method was performed on a phenomenex-luna C18（2）（4.6 mm × 250 mm,5 μm） at 40 ℃ and detected at 280 nm.The mobile phase consisted of acetonitrile-0.1% phosphoric acid（45∶ 55） with a flow rate of 1.0 mL.min-1.Result： The linear calibration curves were obtained in the concentration range of 0.141 69-14.169 mg.L-1 for cinnamaldehyde and 1.066 04-106.604 mg.L-1 for engenol.The average recoveries of cinnamaldehyde and engenol were 102.98%（RSD 1.33%） and 95.40%（RSD 0.40%）.Conclusion： The method is simple,effective,accurate and reproducible for the determination of cinnamaldehyde and eugenol in Goupi Gao.

关 键 词：狗皮膏 桂皮醛 丁香酚 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]