养精种玉颗粒的定性定量方法研究  

Study on Qualitative and Quantitative Methods of Yangjingzhongyu Granules

作　　者：王婴[1] 张婧[2] 王岩[2] 马红霞[3] 

机构地区：[1]广东药学院医药化工学院,广东中山528458 [2]广东药学院中药学院,广州510006 [3]广州医学院第一附属医院,广州510120

出　　处：《中国实验方剂学杂志》2013年第15期76-78,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81273786);广东省科技计划项目(粤科规划字[2011]97号);中山市科技计划项目(2009H019)

摘　　要：目的:建立养精种玉颗粒的定性定量方法。方法:采用薄层色谱法对制剂中的白芍、当归、山茱萸进行鉴别;采用HPLC法测定芍药苷的含量。结果:薄层色谱斑点清晰,阴性对照无干扰;芍药苷进样量在60.56～605.6 ng与峰面积的线性关系良好(r=0.999 6),平均回收率99.2%(RSD 1.5%)。结论:建立的方法简便、准确、重复性好,可用于该制剂的质量控制。Objective： To establish the qualitative and quantitative methods for Yangjingzhongyu Granules.Method： Radix Paeoniae Alba,Angelica sinensis and Fructus corni in the preparation were identified by TLC,and the content of paeoniflorin was determined by HPLC.Result： The TLC spots were clear without interference of the other constituents in the preparation.The linear range of paeoniflorin was 60.56-605.6 ng（r = 0.999 6）.The average recovery was 99.2%（RSD 1.5%）.Conclusion： The method is easy,accurate and reproducible.It can be used for quality control of the preparation.

关 键 词：养精种玉颗粒 鉴别 含量测定 芍药苷 

分 类 号：R284.1[医药卫生—中药学]