UPLC同时测定银黄颗粒中4种黄酮类成分含量  

Simultaneous Determination of Four Flavones in Yinhuang Granule by UPLC

作　　者：乔东鸽[1] 吉丽娜[2,3] 何希荣[2] 冯伟红[2,3] 李东影[3,4] 

机构地区：[1]河南省中医院,郑州450002 [2]中国中医科学院中药研究所,北京100700 [3]中药质量控制技术国家工程实验室,北京100700 [4]天津中医药大学,天津300193

出　　处：《中国实验方剂学杂志》2013年第15期102-105,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2009ZX09301-005;2009ZX09308-003)

摘　　要：目的:建立银黄颗粒中黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的UPLC含量测定方法。方法:采用ACQUITYUPLC H-Class超高效液相色谱仪,ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm);流动相甲醇-0.2%磷酸水溶液,梯度洗脱,流速1 mL.min-1,检测波长274 nm,柱温30℃。结果:黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的线性范围分别为1.09～21.8,0.340～6.80,0.116～2.32,0.102～2.04μg,加样回收率分别为100.8%(RSD 0.32%),97.1%(RSD 0.95%),101.5%(RSD 1.6%),98.7%(RSD 0.059%)。结论:该方法简便、快速,分离效果好,可为全面控制银黄颗粒的质量提供参考。Objective： To develop an UPLC method for determining the content of four flavones in Yinhuang granule.Method： The analysis was performed on an ACQUITY UPLC BEH C18column（2.1 mm × 50 mm,1.7 μm） eluted with methanol and water containing 0.2% phosphoric acid as mobile phases in a linear gradient mode.The flow rate was set at 1.0 mL.min-1,the column temperature was set at,and the detection wavelength was set at 274 nm.Result： The linear ranges were 1.09-21.8 μg for baicalin,0.340-6.80 μg for wogonoside,0.116-2.32 μg for baicalein,0.102-2.04 μg for wogonin respectively.The average recoveries of the four flavones were 100.8%（RSD 0.32%）,97.1%（RSD 0.95%）,101.5%（RSD 1.6%）,98.7%（RSD 0.059%） respectively.Conclusion： The method appeared to be simple,accurate and reliable,which can be used for quality control of the four flavones in Yinhuang granule.

关 键 词：高效液相色谱 银黄颗粒 黄芩苷 汉黄芩苷 黄芩素 汉黄芩素 

分 类 号：R284.1[医药卫生—中药学]