热处理前后壮药“国虾薄”中皂苷类化合物研究  

Saponins from Zhuang-medicine Gocaekmbaw before and after Heat-processing

作　　者：刘慧敏[1] 陈道金[1] 朴香兰[1] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院,北京100081

出　　处：《中国实验方剂学杂志》2013年第15期106-110,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30973960;81011140347;81181240306;81274186)

摘　　要：目的:分离、鉴定热处理前后国虾薄中皂苷类化合物。方法:国虾薄在温度125℃、压力0.24 MPa的条件下,加热处理3 h,用80%乙醇加热回流提取3 h,通过大孔树脂HP-20、硅胶柱及反相柱色谱等分离手段对热处理前后的国虾薄成分进行分离,并用核磁共振波谱、离子阱飞行时间质谱(LCMS-IT-TOF)等数据鉴定其成分。结果:从壮药国虾薄原药材中分离得到2个达玛烷类皂苷成分,经热处理后得另外4个达玛烷类皂苷成分,分别鉴定为绞股蓝皂苷gypenoside XLVI,gypenosideLVI,gypenoside L,gypenoside LI,damulin B和damulin A。结论:热处理能够使国虾薄中达玛烷型皂苷gypenoside XLVI,gypenoside LVI的20位上连有的糖链被水解,为国虾薄热处理产物的成分变化提供实验依据。Objective： Isolation and identification of saponins from Gocaekmbaw before and after heatprocessing was studied.Method： The leaves of Gocaekmbaw were steam-heated at 125 ℃ and 0.24 MPa for 3 h,then extracted with 80% ethanol for 3 h.The extracts were isolated with chromatography using HP-20,silica gel and reversed ODS column.The constituents isolated from Gocaekmbaw before and after heat-processing were identified with 1H-NMR,13 C-NMR and LCMS-IT-TOF spectra.Result： Two dammarane-type saponins were isolated from the original Gocaekmbaw.And four different compounds were got from the heat processed Gocaekmbaw.They were identified as gypenoside XLVI,gypenoside LVI,gypenoside L,gypenoside LI,damulin B and damulin A,respectively.Conclusion： Sugar chains in C20 position of gypenoside XLVI and gypenoside LVI were hydrolyzed by heat-processing to change the constituents of Gocaekmbaw.

关 键 词：国虾薄 热处理 达玛烷类皂苷 

分 类 号：R284.1[医药卫生—中药学]