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机构地区:[1]太原科技大学化学与生物工程学院,太原030021 [2]山西医科大学药学院,太原030001
出 处:《中国实验方剂学杂志》2013年第15期127-129,共3页Chinese Journal of Experimental Traditional Medical Formulae
摘 要:目的:建立消炎止咳片中吗啡的HPLC测定方法。方法:采用高效液相色谱法,Sinochrom-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1 mol.L-1磷酸二氢钾溶液-甲酸(7.5∶92.5∶0.1),流速0.7 mL.min-1,检测波长284 nm。结果:吗啡在42.40~424.0 mg.L-1线性关系良好(r=0.999 9),平均加样回收率为100.25%,RSD 1.67%。结论:采用HPLC测定吗啡,方法简单、灵敏、准确,可用于消炎止咳片中吗啡的含量测定。Objective: To establish a method of high performance liquid chromatography(HPLC) for the determination of morphine in Xiaoyanzhike Tablets.Method: The method of high performance liquid chromatography was carried out on a column of Sinochrom-C18 using the mobile phase of acetonitrile-0.1 mol.L-1 potassium dihydrogen phosphate solution-methanoic acid(7.5∶ 92.5∶ 0.1) with a flow rate of 0.7 mL.min-1.The detection wavelength was set at 284 nm.Result: The calibration curve of morphine was linear over the range of 42.40-424.0 mg.L-1(r = 0.999 9).The average recovery was 100.25%(RSD 1.67%).Conclusion: The method is simple and feasible,which can be used to determine the content of morphine in Xiaoyanzhike Tablets.
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