葛根素、齐墩果酸及其配伍对T2DM大鼠氧化应激和炎症反应的影响  

Regulation of Puerarin and Oleanolic Acid on Antioxidant Activity and Suppressing Inflammatory Reaction in Rats with Type 2 Diabetes Mellitus

作　　者：王会敏[1] 田炜[1] 喇孝瑾[1] 高秀娟[1] 韩婷[1] 王亚[1] 田震亚[1] Hegyi Gabriella 陈震 李继安[1] 

机构地区：[1]河北联合大学,河北唐山063000 [2]匈牙利佩奇大学,佩奇999024 [3]匈牙利东方国药集团,佩奇999024

出　　处：《中国实验方剂学杂志》2013年第15期174-177,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部2011-2012年度中国与匈牙利政府间科技合作项目(2010-5-8);唐山市科技局项目(08360202A-8)

摘　　要：目的:观察葛根素、齐墩果酸及其配伍对2型糖尿病大鼠血糖、血脂、血清超氧化物歧化酶(SOD)、丙二醛(MDA)、糖基化终末产物(AGEs)、超敏C反应蛋白(hs-CRP)及胰腺病理组织学的影响。方法:以高糖、高脂饮食联合链脲佐菌素(30 mg.kg-1)ip法建立2型糖尿病大鼠模型,随机分为模型(M)组、二甲双胍(D)组(100 mg.kg-1.d-1)、葛根素(P)组(30 mg.kg-1.d-1)、齐墩果酸(OA)组(10 mg.kg-1.d-1)、葛根素+齐墩果酸(POA)组(40 mg.kg-1.d-1),同期设正常对照组(K)。各治疗组分别ig给予相应药物治疗。治疗前及治疗2,4,6周分别测定各组大鼠空腹血糖(FBG)及餐后2 h血糖(P2hPG),并于给药6周后,测定大鼠总胆固醇(CHOL)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),hs-CRP,血清SOD,MDA,AGEs水平,光镜下观察胰腺病理组织学变化。结果:M组大鼠FBG,P2hPG,CHOL,TG,LDL-C,hs-CRP,MDA,AGEs较K组升高,HDL-C和血清SOD水平明显下降,有统计学差异,对胰腺胰岛细胞有一定损伤。各治疗组大鼠FBG,P2hPG,CHOL,TG,LDL-C,hs-CRP,MDA,AGEs较M组降低,HDL-C和血清SOD水平增加,对胰腺胰岛细胞损伤有不同程度改善。POA组LDL-C水平较P组、OA组降低明显(P<0.05),对胰腺胰岛细胞损伤明显改善。结论:葛根素、齐墩果酸及其配伍可改善实验性2型糖尿病大鼠的糖、脂代谢,其作用机制可能与抗氧化、抑制炎症反应有关。其中葛根素配伍齐墩果酸在降低LDL-C水平、改善胰岛细胞结构方面优于单纯葛根素、齐墩果酸。Objective： To observe effects of puerarin and oleanolic acid on blood glucose,blood lipid,superoxide dismutase（SOD）,malondialdehyde（MDA）,advanced glycation end products（AGEs）,serum hypersensitive C-reactive protein（hs-CRP） and pancreatic histopathology in rats with type 2 diabetes mellitus（T2DM）,and to approach their pharmacological mechanism.Method： T2DM rats induced by high calories diet and intraperitoneal injection of streptozotacin（STZ） were randomly divided into five groups： diabetes mellitus（M） group,melbine（D） group,puerarin（P） group,oleanolic acid group（OA） and puerarin with oleanolic acid（POA） group.Fasting blood glucose（FBG） and 2-hour postprandial blood glucose（P2hPG） of rats in each group were measured before treatment and after 2,4,6 weeks of administration.After 6 weeks,blood simples were collected for measurement on blood glucose,as well as following indexes,cholesterol（CHOL）,triglyceride（TG）,HDL-cholesterol（HDL-C）,LDL-cholesterol（LDL-C）,hs-CRP,SOD,MDA and AGEs.Pathological changes were observed under light microscope by HE staining.Result： All indexes were inhibited to certain degrees in every treatment group except HDL-C and SOD.The level of LDL-C in POA group decreased more significantly than that in the P group and OA group（P 0.05）,meanwhile,the injury pancreas tissue was improved significantly.Conclusion： The puerarin and oleanolic acid could markedly improve glucose and lipid metabolism in experimental type 2 diabetic rats.It may prove that puerarin with oleanolic acid can relieve the degree of oxidative stress reaction through antioxidant activity and suppressing inflammatory reaction.In terms of decreased LDL-C and improvement of the structure of islet cells,the effects in POA group are more stronger than those in P and OA group.

关 键 词：2型糖尿病 葛根素 齐墩果酸 炎症反应 氧化应激 血糖 血脂 

分 类 号：R285.5[医药卫生—中药学]