四逆散含药血清对大鼠皮层神经元GABA_A受体介导Cl^-电流的影响  

Effect of Sini San Containing Serum on GABA_A-Cl^- Current of Cortical Neurons in Rats

作　　者：刘晓萌[1] 李峰[1] 马捷[1] 刘晶[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中国实验方剂学杂志》2013年第15期202-205,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市自然科学基金项目(7112071)

摘　　要：目的:研究四逆散改善失眠作用的机制。方法:四逆散浸膏按21.7 g.kg-1.d-1给SD大鼠连续灌胃7 d,腹主动脉取血,分离血清,按10%比例加进原代培养SD大鼠皮层神经元培养基,37℃孵育3 h,应用全细胞膜片钳方法,记录四逆散含药血清对原代培养大鼠皮层神经元γ-氨基丁酸A型受体(GABAA)介导的氯离子(Cl-)电流的影响。结果:给予0.3,3,30,300μmol.L-1γ-氨基丁酸刺激,经10%四逆散含药血清作用3 h的皮层神经元,γ-氨基丁酸浓度-效应曲线左移,半效激活浓度降低(P=0.05),氯离子电流的最大峰值(P<0.05)、快速衰减时间常数(τ2,P<0.05)和慢速衰减时间常数(τ1,P<0.05)明显增大。结论:四逆散作用于皮层神经元的γ-氨基丁酸A型受体,调节其介导的氯离子电流,可能是其改善失眠作用的机制之一。Objective： To investigate the mechanism underlying the insomnia-treating effect by Sini San.Method： SD rats were ig given 21.7 g.kg-1.d-1Sini San for 7 d.Serum from the abdominal aorta was collected and added into rats cortical neurons at the final concentration of 10%,cultured at 37 ℃ for 3 h.Whole-cell recording technique was used to observe the γ-aminobutyric acid A（GABAA） receptor activated Cl-flux（IGABA） of rats cortical neurons cultured with the serum from Sini San-treated rats.Result： A series concentration of γaminobutyric acid（GABA） from 0.3-300 μmol.L-1was given,the cortical neurons treated with Sini Sancontaining serum for 3 h resulted in left shift of GABA concentration-response curve and half maximal effective concentration（EC50） was decreased（P =0.05）.Also the peak amplitude（P 0.05）,τ1（P 0.05） and τ2（P 0.05） of IGABA significantly increased.Conclusion： Sini San affects the IGABA of cortical neurons and this may be contributed to the insomnia-treating effect of Sini San.

关 键 词：四逆散 γ-氨基丁酸A型受体 失眠 膜片钳 皮层 

分 类 号：R285.5[医药卫生—中药学]