17-甲氧基-7-羟基-苯并呋喃查尔酮对心肌细胞内游离钙浓度及L-型钙电流的影响  

Effects of 17-methoxyl-7-hydroxy-benzfuranchalcon on Intracellular Calcium Overload in Cultured Cardiac Myocytes of Neonatal Rats Injured by H_2O_2 and L-type Calcium Current in Isolated Ventricular Myocytes of Mice

作　　者：李映新[1] 黄媛恒[2] 覃斐章[1] 林兴[3] 黄仁彬[1] 

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西医科大学实验生理科学实验中心,南宁530021 [3]广西医科大学医学科学实验中心,南宁530021

出　　处：《中国实验方剂学杂志》2013年第15期213-217,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究与技术开发计划项目(桂科攻0630002-2A);广西中医药科技专项课题(GZKZ10-122);2011年广西研究生创新计划项目(2011105981002D26)

摘　　要：目的:研究17-甲氧基-7-羟基-苯并呋喃查尔酮(YLSC)对H2O2诱导的心肌细胞内钙超载的拮抗作用及对L型钙电流(ICa-L)的影响。方法:采用SD大鼠乳鼠进行心肌细胞培养,实验分为①正常对照组;②H2O2组:上机前加入终浓度为0.3 mmol.L-1的H2O2;③预先给予低、中、高不同终浓度YLSC药物处理组:分别给予100,200,400μmol.L-1YLSC的无血清培养基,孵育24 h,上机前加入终浓度为0.3 mmol.L-1H2O2,以Fluo-3/AM荧光指示剂负载,应用激光共聚焦显微镜技术,分别于加入H2O2后15 min内检测细胞内[Ca2+]i的变化;分离昆明种小鼠单个心室肌细胞,全细胞膜片钳技术记录YLSC对ICa-L的影响。结果:①与正常对照组比较,H2O2诱导的模型组细胞内[Ca2+]i增加60.43%±7.75%,而高、中、低YLSC预处理组[Ca2+]i分别增加38.39%±13.87%,14.49%±2.94%,-28.1%±1.52%,与模型组比较显著降低(P<0.01)。②YLSC可使心室肌细胞ICa-L的电流-电压(I-V)关系曲线上移,能改变ICa-L的激活和失活特征,使ICa-L的激活曲线和稳态失活曲线左移。结论:YLSC对心肌细胞钙离子通道有较好的阻断作用,能显著减轻H2O2诱导的心肌细胞内[Ca2+]i超载。Objective： To investigate the effects of 17-methoxyl-7-hydroxy-benzfuranchalcon（YLSC） on intracellular calcium overload in cultured cardiac myocytes of neonatal rats injured by H2O2and L-type calcium current（ICa-L） in isolated ventricular myocytes.Method： The cells of primary cultured cardiac myocytes of neonatal rats were divided into groups： ① control group;② model group： administrated with 0.3 mmol.L-1 H2O2;③YLSC treated groups： incubation respectively with 100,200,400 μmol.L-1YLSC for 24 h,and add 0.3 mmol.L-1 H2O2.Laser confocal microscopy was used with Fluo-3 / Am as indicator to detect changes of [Ca2 ＋]i immediately and 15 mintues after H2O2 intervention.Single ventricular cell was obtained enzymatically by Langendorff perfusion apparatus.The wholecell patch clamp was used to record the ICa-L.Result： ①The average fluorescence intensity values of the model group treated by H2O2 higher significantly than control group and the fluorescence enhancement ratio was 60.43% ± 7.75%.The YLSC could decline the intensity values in a doesdependent manner manner,the fluorescence enhancement ratios of low,middle and high does respectively were 38.39% ± 13.87%,14.49% ± 2.94%,-28.1% ± 1.52%,and all of them were much lower compared with H2O2 group（P 0.01）.② YLSC can up-shifted the current-voltage（I-V） curves and markedly shifted the steady state activation and inactivation curve of ICa-L to the left.Conclusion： YLSC inhibited the L-type calcium channel,it can significantly reduce the myocardial intracellular Ca2 ＋ overload induced by H2O2.

关 键 词：17-甲氧基-7-羟基-苯并呋喃查尔酮 激光扫描共聚焦 细胞内钙浓度 全细胞膜片钳 L型钙电流 

分 类 号：R285.5[医药卫生—中药学]