血必净注射液对宫内感染致早产脑损伤仔鼠脑TNF-α和GFAP表达的影响  

Influence of Xuebijing Injection on Expression of GFAP and TNF-α During Cerebral Damage in the Prematured Neonatal Rat Induced by Intrauterine Infectionn

作　　者：马丙祥[1,2] 党伟利[1] 

机构地区：[1]河南中医学院第一附属医院,郑州450000 [2]南京中医药大学,南京210023

出　　处：《中国实验方剂学杂志》2013年第15期272-275,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省教育厅自然科学研究计划项目(2010B360011)

摘　　要：目的:观察血必净注射液对宫内感染致早产脑损伤仔鼠脑组织肿瘤坏死因子(TNF-α)和胶质纤维酸性蛋白(GFAP)表达的影响。方法:①12只孕第16天脂多糖(LPS)组大鼠予LPS 450μg.kg-1,ip,连续2 d,8只生理盐水组孕鼠等量生理盐水ip。孕22 d前分娩的仔鼠为早产仔鼠。随机选取生理盐水组足月产仔鼠8只作为空白对照组和LPS组早产仔鼠24只。LPS组仔鼠随机分为3组,每组8只,分别为血必净高、低剂量(4,2 g.kg-1)组,模型组。7日龄时开始分别给予血必净注射液或生理盐水,ip,共14 d。②21日龄时对4组大鼠进行神经行为学检测(悬吊试验),并取脑组织,用免疫组化方法测定GFAP,TNF-α水平。结果:与模型组比较,血必净注射液高、低剂量组干预治疗后脑损伤仔鼠行为学方面得到改善(P<0.05);与模型组比较,血必净注射液高、低剂量组干预治疗后,早产脑损伤仔鼠脑组织TNF-α阳性表达减少而GFAP阳性表达增加(P<0.05)。结论:化瘀解毒中药血必净注射液可改善宫内感染所致早产脑损伤,其作用机制可能与降低脑组织TNF-α表达和使GFAP表达升高有关。Objective： To study the influence of Xuebijing injection on expression of glial fibrillary acidic protein（GFAP） and tumour necrosis factor-α（TNF-α） during lipopolysaccharide-induced white matter damage in the prematured neonatal rat induced by intrauterine infection.Method： Twelve rats with pregnancy duration of 16 days were intra-peritoneally injected LPS 450 μg.kg-1 for 2 day,and another 8 similar rats were injected with normal saline as the controls.The premature delivery was difined as a delivery before 22 days of pregnancy.Eight normal born rats from the controls were selected as the blank control group,and 24 premature delivered rats induced by LPS were selected as the models.The models were then divided into 3 groups： the model group,Xuebijing 4 g.kg-1 and Xuebijing 2 g.kg-1 group,ip,for 14 d.The neurobehavior of rat was observed by suspention test.The expressin of GFAP and TNF-α were detected in brain sections by immunohistochemistry.Result： Compared with the Blank model control,Xuebijing low dose and high dose could improve the neurobehavior in rats（P 0.05）;Compared with the model control group,expression of TNF-α was decreased and GFAP was increased in both dose groups of Xuebijing（P 0.05）.Conclusion： Xuebijing injection can treat intrauterine infection-induced white matter damage in the prematured neonatal rat,the mechanism may be related to removal of TNF-α expression and increase in GFAP expression.

关 键 词：脑性瘫痪 宫内感染 脑白质损伤 胶质纤维酸性蛋白 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]