朱砂、含朱砂复方对人肝细胞的毒性对比研究  

Cytotoxicity of Cinnabar and Cinnabar-containing Compounds in Human Liver HL-7702 Cells

作　　者：付中祥[1] 时京珍[1] 刘杰[2] 张永萍[3] 

机构地区：[1]贵阳中医学院实验中心基础医学实验室,贵阳550002 [2]遵义医学院贵州省基础药理重点实验室,贵州遵义563003 [3]贵阳中医学院药物制剂实验室,贵阳550002

出　　处：《中国实验方剂学杂志》2013年第15期285-289,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省国际科技合作计划项目(黔科合外G字【2011】7020号);九种名优中药品种的技术提升与深度开发(黔科合重大专项字【2011】6019)

摘　　要：目的:对比研究朱砂、几种含朱砂复方与其他汞存在形式对HL-7702人肝细胞的毒性。方法:分别以朱砂、朱砂安神丸、廖元和堂化风丹、万胜化风丹、硫化汞、氯化汞、甲基汞加入已接种于96孔板的HL-7702人肝细胞后,检测各组的细胞存活率、检测各组细胞内、外的汞含量。结果:等汞含量和等汞浸出量的朱砂、朱砂安神丸、廖元和堂化风丹、万胜化风丹和硫化汞对HL-7702人肝细胞的存活率与正常组无明显差异,与等汞含量和等汞浸出量的氯化汞和甲基汞均有明显差异(P<0.05);分别以等汞含量、等汞浸出量的朱砂、朱砂安神丸、廖元和堂化风丹、万胜化风丹和硫化汞培养的HL-7702人肝细胞内、外汞含量均低于氯化汞、甲基汞(P<0.05)。结论:等汞含量、等汞浸出量的朱砂、朱砂安神丸、廖元和堂化风丹和万胜化风丹对人肝HL-7702细胞的毒性远小于对应等汞含量、等汞浸出量的氯化汞、甲基汞。Objective： To study the toxicity of cinnabr,Zhusha Anshen Wan,Liaoyuan Hetang Huafeng Dan,Wansheng Huafeng Dan,HgS,HgCl2 and MeHg in human liver HL-7702 cells.Method： Cultured cells were treated with cinnabar containing compounds and mercurial for 24 h,and was determined by the MTS assay,extracellular and intracellular Hg contents were determined by atomic fluoresence detector.Result： The cytotoxicity of cinnabr,Zhusha Anshen Wan,Liaoyuan Hetang Huafeng Dan,Wansheng Huafeng Dan,HgS had a obvious difference compared with HgCl2 and MeHg is same as the intracellular and extracellular mercury accumulation.Conclusion： The toxicity of cinnabr,Zhusha Anshen Wan,Liaoyuan Hetang Huafeng Dan,Wansheng Huafeng Dan,HgS with same mercury accumulation and same mercury dissolution is extremely lower than the corresponding HgCl2 and MeHg in human liver HL-7702 cells.

关 键 词：朱砂 含朱砂复方 HL-7702人肝细胞 毒性 

分 类 号：R285.5[医药卫生—中药学]